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Genetic mapping of resistance to southern corn rust (Puccinia polysora underw.) of maize

KrishiKosh

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Title Genetic mapping of resistance to southern corn rust (Puccinia polysora underw.) of maize
 
Creator P, Bhavani
 
Contributor K. M, Harinikumar
 
Subject genes, diseases, maize, chromosomes, genetics, planting, plant oils, yields, genomes, proteins
 
Description Southern corn rust (SCR) is a major disease of maize whose periodic
nature has rendered breeding for resistance a challenging task. There is a need
to identify tightly linked molecular markers to enhance breeding efficacy. F2:3
mapping population derived by crossing two inbreds namely SKV50 (resistant)
with CM202 (susceptible) was used in the study. The population manifested
high genotypic and phenotypic variability for grain yield and SCR resistance.
Grain yield, cob length and SCR resistance exhibited high heritability coupled
with GA percent of mean. Chi square analysis revealed dominance inheritance
of the gene governing resistance to SCR. An integrated map for bin 10.01
involving genetic elements Rpp9, RppQ, RppD and linked SSR & BACs within
the contig 392 was developed using Maize GDB. Genes identified within the
contig using Fgenesh (in bits of 1 MB) were subjected to BLAST. Orthologs loci
manifesting high degree of similarity with genomes of rice, sorghum and
brachypodium grass harbouring genes associated with disease resistance were
obtained. 25 genes were shortlisted for primer designing and further analyses.
Seven SSRs flanking the reported resistance genes were also included. Four
genes viz., Pp2 (phytoene synthase), Pp7 (phytosulphokines), Myb2
(transcription factor), Psk (phytosulphokines) displayed polymorphism between
parents and were subsequently used for screening the F2 mapping population.
Among these, Pp7 and Pp2 showed association with the SCR resistance. In this
study the co-localization of genetic elements of SCR, locus for common rust
resistance and annotated functional genes along with candidate gene primers
Pp7 and Pp2 encoding phytosulfokines and phytoene synthase respectively has
been shown.
 
Date 2016-05-19T13:58:54Z
2016-05-19T13:58:54Z
2013-01-29
 
Type Thesis
 
Identifier Th-10455
http://krishikosh.egranth.ac.in/handle/1/66134
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru