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Role of ACC (1-aminocyclopropane-1-carboxylate) utilizing pseudomonas and mesorhizobium in disease control and plant growth stimulation of chickpea (Cicer arietinum L.)

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Title Role of ACC (1-aminocyclopropane-1-carboxylate) utilizing pseudomonas and mesorhizobium in disease control and plant growth stimulation of chickpea (Cicer arietinum L.)
 
Creator Kiran Kumari
 
Contributor Sindhu, S. S.
 
Subject Chickpea, Nodulation, Mesorhizobium, Pseudomonas, ACC utilization, Fusarium oxysporum, Disease control
 
Description Chickpea is an important commercially utilizable crop and its high yielding varieties are more susceptibile to soil borne fungal phytopathogens i.e., Fusarium oxysporumand Rhizoctonia bataticola. Recently, ACC deaminase containing Mesorhizobium and Pseudomonasbacteria have been found to improve nodulation and suppressed disease in chickpea by lowering the level of stress ethylene. In this, study, 85 Mesorhizobiumisolates and sixty one Pseudomonas isolates were screened for utilization of ACC on Dworkin and Foster’s minimal medium. Frequency of Mesorhizobium and Pseudomonas isolates having the ability to utilize ACC was found 30.6 and 14.3%, respectively. Among ACC + isolates, 7.37% isolates ofMesorhizobiumshowed antifungal activity against F. oxysporumand 6.32% isolates inhibited the growth of R. bataticola.on PDA plates. Screening of Pseudomonas isolates showed that 21.98 and 47.5% isolates inhibited the growth of F. oxysporum and R. bataticola, respectively. Mutant altered in ACC utilization were derived from Mesorhizobium andPseudomonas isolates by using nitrosoguanidine. Seven mutants fromPseudomonasisolate PPBM36 and 5 mutants from Mesorhizobiumisolate CMK39 lacking ACC utilization were obtained. Mesorhizobium isolates i.e., CMK12, CMK39, CRYM13 and CMK21 formed 75 to 98 nodules per plant and 229.3 - 278.5% increase in plant dry weight was observed at 90 days of growth in chillum jar assembly conditions. Coinoculation of Mesorhizobium isolate, CMK39 with Pseudomonas isolate PPBM36 formed 73 nodules per plant and caused 48.4 - 127.5% increase in root and shoot dry weight at 90 days ofgrowth. In Fusarium oxysporuminoculated soil, single inoculation of Mesorhizobiumisolate CMK39 caused 46.5% increase whereas its coinoculation with Pseudomonasisolate PPBM36 further enhanced shoot dry weight upto 67.5% in comparison to uninoculatedcontrol. Disease caused byF. oxysporum was effectively controlled by coinoculation of Mesorhizobium isolate CMK39 with Pseudomonas isolate PPBM36. ACC utilizing Mesorhizobium and Pseudomonas isolates were found more effective in control of disease symptoms as compared to ACC - isolates and mutants. Thus, ACC utilizing Mesorhizobium and Pseudomonas isolates could be used for disease control and plant growth promotion of chickpea under field conditions.
 
Date 2016-08-08T14:34:10Z
2016-08-08T14:34:10Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71557
 
Language en
 
Format application/pdf
 
Publisher CCSHAU