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Characterization of Helicoverpa armigera (Hubner) α-amylase and status of antioxidants in Pigeonpea, Cajanus cajan (Millspaugh)

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Title Characterization of Helicoverpa armigera (Hubner) α-amylase and status of antioxidants in Pigeonpea, Cajanus cajan (Millspaugh)
 
Creator Kaur, Rimaljeet
 
Contributor Gupta, A.K.
 
Subject enzymes, planting, biological phenomena, pigeonpeas, starch, genotypes, acidity, organic acids, inorganic acid salts, biological interaction
 
Description The objectives of present study were to determine the status of antioxidants in
pigeonpea on account of Helicoverpa armigera herbivory, to characterize its gut α-amylase
and to identify some effective non-proteinaceous α-amylase inhibitors. Leaves, developing
seeds and pod wall of nine pigeonpea genotypes (four moderately tolerant, three intermediate
and two moderately susceptible) were used to determine the status of enzymatic and nonenzymatic
antioxidants, nitrate metabolizing enzymes, malondialdehyde, H2O2, nitrate and
nitrite against H. armigera infestation. The increase in activities of polyphenol oxidase,
diamine oxidase, polyamine oxidase, phenylalanine ammonia lyase and tyrosine ammonia
lyase and higher proline, lower malondialdehyde content upon pod borer infestation in
different tissues of moderately resistant genotypes suggested their contribution in providing
resistance to pigeonpea. A lower H2O2 content and higher catalase activity alongwith higher
phenolic content following pod borer attack in moderately resistant genotypes could be
additional factors for providing resistance against H. armigera. In general, higher status of
nitrate reductase, nitrite reductase, nitrite content and zinc content was found in moderately
resistant genotypes than in moderately susceptible genotypes on account of H. armigera
herbivory. Two α-amylases differing in molecular weights viz., ~81 kDa and ~14 kDa were
purified by Sephadex G-100 column chromatography. ZnSO4, FeSO4, CuSO4, NaNO2, citric
acid, oxalic acid, salicylic acid and α-amylase inhibitor (α-AI) from pigeonpea mature seeds
were the potent inhibitors of both amylases. Mature seeds of pigeonpea genotypes have
significantly high α-AI activity against H. armigera amylase in comparison with amylases
from Aspergillus oryzae, porcine pancreas and barley malt.
 
Date 2016-04-26T12:14:27Z
2016-04-26T12:14:27Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65594
 
Language en
 
Format application/pdf
 
Publisher PAU