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Inv A Gene Specific PCR for Detection of Salmonella from Broilers

KrishiKosh

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Title Inv A Gene Specific PCR for Detection of Salmonella from Broilers
Vet. World, 2011, Vol.4(12):562-564
 
Creator Shanmugasamy, Malmarugan
Velayutham, Thenmozhi
Rajeswar, Johnson
 
Subject Salmonella
Broiler
Polymerase Chain Reaction (PCR)
Inv A Gene
 
Description Poultry meat has been identified as one of the principal foodborne source of Salmonella. In this preliminary study the prevalence of Salmonella spp. contamination of broiler carcasses, were determined. Sixty samples were collected from poultry carcasses from the commercial broiler slaughtering facility in Namakkal, Tamil Nadu. The presence of
Salmonella spp in collected samples was assessed by performing the pre-enrichment and enrichment culture, followed by PCR assay. The primers were selected from the invA gene specific for the detection of Salmonella spp. In this study 8.3% of poultry carcasses were found to be contaminated with Salmonella spp. In order to provide a more accurate profile of the prevalence of Salmonella spp in broiler carcasses, it is pertinent to use invA gene specific PCR method that could be considered as an appropriate alternative to conventional culture method.
 
Date 2016-06-11T10:16:16Z
2016-06-11T10:16:16Z
2011-12
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67197
 
Language en
 
Format application/pdf
 
Publisher Veterinary World