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STUDIES ON ANTIOXIDANT ACTIVITY IN FRUITS OF CRAB APPLE

KrishiKosh

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Title STUDIES ON ANTIOXIDANT ACTIVITY IN FRUITS OF CRAB APPLE
 
Creator SHARMA, RUCHIKA
 
Contributor NATH, AMARJIT K.
 
Subject enzymes, apples, fruits, antioxidants, byproducts, extraction, acidity, inorganic acid salts, vitamins, chromatography
Crab apple (Malus baccata),antioxidant enzyme
 
Description ABSTRACT
Crab apple (Malus baccata) fruits of Himalayan region were analyzed for total antioxidant
activity, phenol content, ascorbic acid content and antioxidant enzyme activities viz., superoxide
dismutase, catalase, peroxidase and ascorbate peroxidase. The results obtained were compared with
fruits of commercial apple cultivar Red Delicious (Malus domestica ‘Red Delicious’). Superoxide
dismutase enzyme was purified and characterized from pulp extract of crab apple fruits by ammonium
sulphate precipitation, gel filtration chromatography (Sephadex G-100) and ion exchange
chromatography (DEAE-Sephadex). On DEAE-Sephadex, three isoforms of superoxide dismutase
(SOD) were eluted with water, 0.1M and 0.2 M KCl gradients, thereby indicating different charges on
the isoforms. .The isoforms, superoxide dismutase enzyme-1, superoxide dismutase enzyme-2 and
superoxide dismutase enzyme-3 were purified to 14.1, 8.8 and 10 fold with 16.6, 14.0 and 15.4 per
cent recovery, respectively. The molecular weight of purified isoforms of superoxide dismutase
enzyme-1, superoxide dismutase enzyme-2 and superoxide dismutase enzyme-3 were found to be
15,848; 17,378 and 20,892 daltons, respectively, as revealed by SDS-PAGE. Isoforms of superoxide
dismutase enzyme were found to be heat stable up to 900C. All the three isoforms had pH optima of
7.8. The activity of all the three isoforms of superoxide dismutase enzyme was found to increase upon
incubation with increasing concentrations of zinc (Zn2+) ions.
Superoxide dismutase is a powerful free radical scavenger which has been clinically shown to
protect the brain, skin, heart, spinal cord from ischemic injury and for treatment of the disorders
associated with free radical damage and long-term exposure to toxins like cigarette smoke. Keeping in
view the role of superoxide dismutase enzyme in stress tolerance in plants, there is a need to identify
novel genes of this enzyme from native germplasm.
 
Date 2017-01-20T13:00:50Z
2017-01-20T13:00:50Z
2011
 
Type Thesis
 
Identifier 47324
http://krishikosh.egranth.ac.in/handle/1/97351
 
Language en
 
Format application/pdf