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STUDIES ON GENETIC VARIABILITY OF CUCUMBER ( Cucumis sativus L.) BASED ON MORPHOLOGICAL TRAITS AND MOLECULAR MARKERS

KrishiKosh

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Title STUDIES ON GENETIC VARIABILITY OF CUCUMBER ( Cucumis sativus L.) BASED ON MORPHOLOGICAL TRAITS AND MOLECULAR MARKERS
 
Creator Kalidas Pati
 
Contributor Ravind er Kumar
 
Subject ---
 
Description t-7983
Genetic variability of cucumber (
Cucumis sativus
L.) based on
morphological traits and molecular markers
Abstract
The present investigation was carried
out at the Division of Vegetable
Science, I.A.R.I., New Delhi during Marc
h to June, 2007 to study the genetic
variability of cucumber (
Cucumis sativus
L.) by utilizing 36 genotypes based on
morphological traits and molecular mark
er systems. The genetic parameters
viz
genotypic and phenotypic co-efficient of va
riation, heritability, genetic advance,
correlation, path coefficient and genetic dive
rgence were studied with respect to 12
quantitative traits. PCR based marker, Sequence Tagged Microsatellite Site (STMS)
markers had been utilized for better understand
ing of variability at
molecular level.
Highly significant differences were
observed among the genotypes for all
the characters studied. Yield per plant followed by fruit index exhibited the highest
phenotypic and genotypic coefficient of vari
ation. The high heritability estimates
coupled with high genetic advance for char
acters like fruit index,
yield per plant and
fruit weight may attributed to additive
gene effects and could be improved through
selection. Characters like number of fruits
per plant and fruit weight had a highly
significant positive correlation with yiel
d per plant at genotypic and phenotypic
level. Path analysis at genotypic level re
vealed that fruit weight had the maximum
direct effect on yield per plant followed by
days to opening of first female flower,
total number of fruits per plant.
All the 36 genotypes were grouped into 7 clusters
based on D
2
values, which exhibited no a
ssociation between clusters and
geographical distributions
of the genotypes.
Out of 40 Sequence Tagged Microsatelli
te Site (STMS) markers screened
to study molecular diversity, 17 primer
s showed polymorphism. Morphological
diversity of 36 genotypes based on 12 quan
titative traits and molecular diversity
based on 17 STMS primers were not sim
ilar and the grouping pattern was different
in the two systems, which indicated the role of environment in the expression of
phenotypic traits.
Results obtained from variability st
udies and diversity
analysis through
morphological basis and molecular markers,
suggested the utiliz
ation of promising
genotypes in the breeding programme for the
development of high
yielding varieties
and F
1
hybrids.
 
Date 2017-01-12T11:46:48Z
2017-01-12T11:46:48Z
2008
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/95864
 
Format application/pdf