STUDIES ON GENETIC VARIABILITY OF CUCUMBER ( Cucumis sativus L.) BASED ON MORPHOLOGICAL TRAITS AND MOLECULAR MARKERS
KrishiKosh
View Archive InfoField | Value | |
Title |
STUDIES ON GENETIC VARIABILITY OF CUCUMBER ( Cucumis sativus L.) BASED ON MORPHOLOGICAL TRAITS AND MOLECULAR MARKERS
|
|
Creator |
Kalidas Pati
|
|
Contributor |
Ravind er Kumar
|
|
Subject |
---
|
|
Description |
t-7983
Genetic variability of cucumber ( Cucumis sativus L.) based on morphological traits and molecular markers Abstract The present investigation was carried out at the Division of Vegetable Science, I.A.R.I., New Delhi during Marc h to June, 2007 to study the genetic variability of cucumber ( Cucumis sativus L.) by utilizing 36 genotypes based on morphological traits and molecular mark er systems. The genetic parameters viz genotypic and phenotypic co-efficient of va riation, heritability, genetic advance, correlation, path coefficient and genetic dive rgence were studied with respect to 12 quantitative traits. PCR based marker, Sequence Tagged Microsatellite Site (STMS) markers had been utilized for better understand ing of variability at molecular level. Highly significant differences were observed among the genotypes for all the characters studied. Yield per plant followed by fruit index exhibited the highest phenotypic and genotypic coefficient of vari ation. The high heritability estimates coupled with high genetic advance for char acters like fruit index, yield per plant and fruit weight may attributed to additive gene effects and could be improved through selection. Characters like number of fruits per plant and fruit weight had a highly significant positive correlation with yiel d per plant at genotypic and phenotypic level. Path analysis at genotypic level re vealed that fruit weight had the maximum direct effect on yield per plant followed by days to opening of first female flower, total number of fruits per plant. All the 36 genotypes were grouped into 7 clusters based on D 2 values, which exhibited no a ssociation between clusters and geographical distributions of the genotypes. Out of 40 Sequence Tagged Microsatelli te Site (STMS) markers screened to study molecular diversity, 17 primer s showed polymorphism. Morphological diversity of 36 genotypes based on 12 quan titative traits and molecular diversity based on 17 STMS primers were not sim ilar and the grouping pattern was different in the two systems, which indicated the role of environment in the expression of phenotypic traits. Results obtained from variability st udies and diversity analysis through morphological basis and molecular markers, suggested the utiliz ation of promising genotypes in the breeding programme for the development of high yielding varieties and F 1 hybrids. |
|
Date |
2017-01-12T11:46:48Z
2017-01-12T11:46:48Z 2008 |
|
Type |
Thesis
|
|
Identifier |
http://krishikosh.egranth.ac.in/handle/1/95864
|
|
Format |
application/pdf
|
|