Record Details

Studies on Pathology and Pathogenesis of Mycoplasmosis in Small Ruminants

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Studies on Pathology and Pathogenesis of Mycoplasmosis in Small Ruminants
 
Creator Uma, S.
 
Contributor Narayan Swamy, H.D.
Satyanarayana, M.L.
Jayakumar, K.
Narayan Swamy, M.
Gajendragad, M.R.
Ramachandra, S.G.
 
Subject ---
 
Description Ph.D. Thesis
Thirty four isolates of mycoplasma (29.06%) were obtained from 117 samples
comprising 40 milk (14;35%), 47 nasal swabs (12;25.53%) and 30 lung tissues
(8;26.67%) from pneumonic and mastitic sheep and goats. More number of mycoplasma
isolates was obtained from goats (40.63 %;13/32) than sheep (24.7%; 1/85). The isolates
were confirmed by culture, serological tests and PCR. Mycoplasma agalactiae was most
common isolate (68%;23/34), followed by M. mycoides subsp. mycoides LC (9%;3/34)
and 8 (23%) isolates were unidentified at species level. Protein profiles of M. agalactiae
isolates exhibited immunodominant proteins on immunoblotting with specific HIS.
Molecular characterization of four M. agalactiae isolates by cloning, sequencing and
phylogenetic analysis revealed that the isolates were closely related to the French and
Iranian strains. Sheep and goat serum samples from 12 districts of Karnataka were found
negative for CCPP by slide agglutination test (SAT). Seroprevalence of M. agalactiae
was studied with 306 sheep and 187 goat serum samples and the prevalence was 41.83%
and 43.85% by SAT and 50.97% and 80.75 % by indirect ELISA in sheep and goats
respectively. The sensitivity and specificity of the indirect ELISA compared to SAT was
74.01% and 44.44% respectively. Pathology of mycoplasmosis in field cases was studied.
Pathogenesis of M. agalactiae isolates was studied using rabbit model by intramammary,
intranasal and intraarticular routes. The clinical signs, haematology, gross and
histopathological lesions induced in the different models were studied. An indirect
ELISA was standardized to study the humoral immune response in the infected animals.
M. agalactiae produced an acute infection as early as 1 dpi which proceeded to a chronic
form by 5 dpi. IHC and reisolation studies showed the persistence of the organism till 9
dpi.
 
Date 2016-08-02T13:59:35Z
2016-08-02T13:59:35Z
2011-05-27
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/70560
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar