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Genetic mapping and qtl analysis for disease resistance, yield and quality in groundnut (arachis hypogaea l.)

KrishiKosh

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Title Genetic mapping and qtl analysis for disease resistance, yield and quality in groundnut (arachis hypogaea l.)
 
Creator Sujay V.
 
Contributor M. V. C. Gowda
 
Subject Genetics & Plant Breeding
 
Description Recombinant Inbred Line (RIL) populations of TAG 24 × GPBD 4 (RIL-4) and TG
26 × GPBD 4 (RIL-5) were phenotypically evaluated and genotyped with SSR markers to
construct the linkage map and identify the QTL governing resistance to late leaf spot (LLS)
and rust, yield and quality traits in groundnut. Ample variability was observed for both the
diseases (LLS and rust) however it was low to moderate for morphological and yield related
traits in both the populations.
A total of 3,101 and 3,284 SSR markers were screened on parents of mapping
populations, of which 211 and 210 markers were considered for linkage map construction. A
map of 1982.90 cM with 20 linkage groups (LGs) was obtained for RIL-4 with 190 markers,
while RIL-5 had a map of 1963.40 cM with 21 LGs with 182 marker loci. Consensus map
was developed with 227 SSR loci of which 144 were common between two maps. It had 20
LGs with genome coverage of 1198.76 cM.
QTL analysis with composite interval mapping identified a total of 90 (RIL-4) and 60
(RIL-5) QTLs localized on individual maps. From both the populations, a total of 28 QTLs
for late leaf spot, 14 QTLs for rust, 36 QTLs for morphological traits, 29 QTLs for yield and
43 QTLs for quality were identified. Among the 150 QTLs identified, 55 were major QTLs
and 95 were minor QTLs.
Major QTLs for LLS were identified for the first time in cultivated groundnut and
they need to be validated. Presence of major QTL for rust was reconfirmed, and in addition
to IPAHM103, a few new markers (GM1536, GM2301 and GM2079) linked to rust
resistance were identified and validated in diverse germplasm. Further, FAD2A allele specific
marker has been validated and mapped on the genetic map. Tightly linked markers for rust
and FAD2A allele for oil quality can be deployed simultaneously in marker assisted breeding
in cultivated groundnut.
 
Date 2016-07-27T09:54:07Z
2016-07-27T09:54:07Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69994
 
Format application/pdf
 
Publisher UAS Dharwad