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STUDY OF THE DIFFERENT FACTORS INFLUENCING THE SHELF LIFE OF CARRIER BASED BIOFERTILIZERS

KrishiKosh

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Title STUDY OF THE DIFFERENT FACTORS INFLUENCING THE SHELF LIFE OF CARRIER BASED BIOFERTILIZERS
 
Creator THIRUMAL, G
 
Contributor Subhash Reddy, R
 
Subject bacteria, application methods, rhizobium, polysaccharides, planting, productivity, inorganic acid salts, vermicomposting, biological development, clay minerals
 
Description In the present study a total of ten Rhizobial and fifteen Pseudomonas bacterial
isolates collected from different sources. These cultures were studied morphologically and
biochemically for purity confirmation. All cultures were further screened for their plant
growth promoting properties such as P, K, Zn solubilzation, IAA production and biocontrol
activity (Siderophore production, HCN production).The screening test results revealed that,
among all Rhizobial isolates, GNR-1 showed best plant growth promoting abilities in invitro
conditions. Similarly, among all Pseudomonas isolates, RGP-1 had shown better plant
growth promoting abilities. These promising cultures were selected for main experiment to
evaluate the shelf life of carrier based bioinoculants with different sterilization methods and
different carrier materials stored at different temperatures in different intervals. The
selected efficient PGP Rhizobium (GNR-1) and Pseudomonas (RGP-1) isolates were
multiplied in large quantities in appropriate culture broth by incubating at 28±2°C in an
incubator shaker till they attained log phase with a cell load of l×l09cfu ml-1. In order to
find the efficient sterilization method (autoclave and irradiation) of carrier materials were
followed. For biofertilizer production Vermicompost, Vermiculate, Lignite, and Sodium
Alginate carriers were used for bioinoculant preparation and stored at different
temperatures i.e. 4ºC and 28ºC.
For this experiment, Factorial CRD with treatments of T1:S1C1O1(Autoclaved
Vermicompost with Rhizobium spp), T2:S1C1O2(Autoclaved Vermicompost with
Pseudomonas spp), T3:S2C1O1(Irradiated Vermicompost with Rhizobium spp), T4: S2C1O2
(Irradiated Vermicompost with Pseudomonas spp), T5:S1C2O1(Autoclaved Sodium alginate
with Rhizobium spp), T6:S1C2O2(Autoclaved Sodium alginate with Pseudomonas spp),
T7:S2C2O1(Irradiated Sodium alginate with Rhizobium spp),T8:S2C2O2(Irradiated Sodium
alginate with Pseudomonas spp), T9:S1C3O1(Autoclaved Lignite with Rhizobium spp,
T10:S1C3O2 (Autoclaved Lignite with Pseudomonas spp),T11:S2C3O1(Irradiated Lignite
Author : G. THIRUMAL
Title of the thesis : STUDY OF THE DIFFERENT FACTORS
INFLUENCING THE SHELF LIFE OF
CARRIER BASED BIOFERTILIZERS
Degree : MASTER OF SCIENCE IN AGRICULTURE
Faculty : AGRICULTURE
Discipline AGRICULTURAL MICROBIOLOGY AND
BIOENERGY
Major Advisor : Dr. R. SUBHASH REDDY
University PROFESSOR JAYASHANKAR
TELANGANA STATE AGRICULTURAL
UNIVERSITY
Year of submission : 2016
with Rhizobium spp), T12:S2C3O2(Irradiated Lignite with Pseudomonas spp), T13:S1C4O1
(Autoclaved Vermiculite with Rhizobium spp), T14:S1C4O2(Autoclaved Vermiculite with
Pseudomonas spp),T15:S2C4O1(Irradiated Vermiculite with Rhizobium spp), T16:S2C4O2
(Irradiated Vermiculite with Pseudomonas spp). The carrier based microbial inoculants
prepared with different carrier material was kept in different temperature levels viz., Room
temperature (28±2ºC) and Refrigerator (4°C). The surviving populations of PGPB at
different temperatures were determined and population was enumerated by dilution plate
technique at different intervals i.e monthly upto 8 months.
Survival rate of rhizobial and Pseudomonad cells was more upto 8th month at 4ºC
compared to 28±2ºC. This results revealed that 4ºC storage temperature is best suitable for
storage of carrier based inoculants because of low level of moisture content in the the
carrier inoculants stored at 28±20C temperature. Though the bacterial population decreased
from 1st month to 8th month the survival rate of rhizobial and Pseudomonad cells was more
upto 8th month at 40C. In the view of comparison of both sterilization methods, irradiation
sterilized carrier based inoculants showed good survival rate compared to autoclave
sterilization method. Similarly with respect to evaluation of shelf life of bacterial cells in
different carrier materials like sodium alginate vermicompost lignite and vermiculite,
sodium alginate based inoculants maintained good population count upto 8th month. Overall
results revealed that sodium alginate based bioinoculants under irradiation sterilization,
maintained more viable count upto eights months.
 
Date 2017-01-03T10:27:35Z
2017-01-03T10:27:35Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94069
 
Language en
 
Relation D10023;
 
Format application/pdf
 
Publisher PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY. HYDERABAD