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EVALUATION AND CHARACTERIZATION OF GROUNDNUT TRANSGENICS (Arachis hypogaea) CO-EXPRESSING THREE TRANSGENES

KrishiKosh

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Title EVALUATION AND CHARACTERIZATION OF GROUNDNUT TRANSGENICS (Arachis hypogaea) CO-EXPRESSING THREE TRANSGENES
 
Creator SHEELA, H S
 
Contributor CHANDRASHEKAR REDDY, P
 
Description For crops grown under rainfed conditions, besides water mining, intrinsic cellular level
tolerance (CLT) mechanisms to sustain the cell metabolism is important. Even with
higher water mining abilities under drought, plants experience decreased water status
hence improving CLT mechanisms is important. The better plant adaptation to drought
can be achieved through improving some of plant characteristics like, improving of crop
variety root characteristics and CLT mechanisms. Genes which regulates the activity of
cellular chaperones and other mechanisms associated with sustainable transcription and
translational machinery are relevant. In the present study OsAlfin1a root associated gene
and heat shock transcription factor (PgHSF4) and salinity tolerant PDH45 involved in
protein turnover and protection were co-expressed in groundnut to improve root growth
and cellular level tolerance. Multigene coexpressing vector with
PDH45:PgHSF4:OsALFIN1 was developed using modified gateway cloning technology.
Transgenics were analysed for gene integration by PCR and RT-PCR. The T3 generation
transgenics showed improved adaptability when exposed to midseason moisture stress. A
few transgenics showed cooler canopy temperature compared to wild type plants.
Transgenics showed improved tolerance to salinity and ethrel stress induced by maintain
high leaf chlorophyll content and less leakage of solutes. Transgenics plants showed
increased plant height, improved pod dry weight and higher TDM under stress
conditions. The transgenics which maintained cooler canopy temperatures also showed
improved root growth compared to wild type. When exposed to water deficit condition,
superior transgenics lines were identified with improved tolerance to drought, salinity and
high temperature with improved productivity under water limited conditions.
 
Date 2016-11-30T08:31:06Z
2016-11-30T08:31:06Z
2012-08-02
 
Type Thesis
 
Identifier TH-10264
http://krishikosh.egranth.ac.in/handle/1/88275
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore