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Isolation and evaluation of Streptomyces lavendulae MTCC 706 for its nematicidal potential against root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919).

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Title Isolation and evaluation of Streptomyces lavendulae MTCC 706 for its nematicidal potential against root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919).
Ph.D.
 
Creator C SELLAPERUMAL
 
Contributor Kamra, Anju
 
Subject Isolation and evaluation of Streptomyces lavendulae MTCC 706 for its nematicidal potential against root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919)
Isolation and evaluation of Streptomyces lavendulae MTCC 706 for its nematicidal potential against root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919)
 
Description Title: Isolation and evaluation of Streptomyces lavendulae MTCC 706 for its nematicidal potential against root-knot nematode, Meloidogyne incognita (Kofoid and White, 1919)
Among the sixteen isolates of Streptomyces from compost samples collected from different locations, isolate 16 showed the highest antagonistic effects against root-knot, Meloidogyne incognita juveniles (J2s) and was identified as S. lavendulae MTCC 706. Its cell free filtrate collected at 13 days of incubation caused an average immobility of 85.2 and mortality 71.2 against M. incognita J2 within 72 h with 88.9% hatch inhibition in the standard extract.
Ethyl acetate extracts (EAE) of S. lavendulae MTCC 706 showed an average per cent immobility and mortality of 82.6 and 80.1, respectively which was significantly more than 57.5 and 45.9 observed in hexane extract (HE). The bioefficacy of the EAE and HE on egg hatch bioassays recorded upto 21 days, too indicated that the nematicidal moieties were more soluble in the polar solvent than in the non polar solvent; an average of 97.8% inhibition was observed in EAE and 41.8 in HE in the standard extract, although inhibition was observed in all 6 dilutions of the extracts. The effects on immobility, mortality and hatch inhibition were positively related to the concentrations tested in all the above treatments.
The drench application of S. lavendulae MTCC 706 @ 2% v/w (SLT) resulted in less than 50% juvenile invasion of M. incognita J2s in tomato (cv. Pusa Ruby), delayed development of J4s, 50.7% reduction in root galling and 42.7% reduction in eggmass production, compared to control. The reproduction factor was 1.2 in SLT, compared to 2.4 in control in 45 days. SLT was compatible with the nematicide carbofuran, as increase in bioefficacy of SLT was observed with 60% reduction in root galling, 61.5% reduction in eggmass production, and a reproduction factor of 1.1 in the combined application with carbofuran @ 0.5Kg a.i./ha.
Replanting of tomato in the pretreated soil revealed a further reduction in root galling (81.3%), eggmass production (80.5%) and reproduction factor (0.6), indicating establishment of the actinomycete in the soil.
 
Date 2016-03-08T15:42:12Z
2016-03-08T15:42:12Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64940
 
Language en_US
 
Format application/pdf
 
Publisher IARI, DIVISION OF NEMATOLOGY, NEW DELHI