Genetics of Maydis leaf blight resistance and evaluation of hybrids for yield and quality traits in Maize
KrishiKosh
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Title |
Genetics of Maydis leaf blight resistance and evaluation of hybrids for yield and quality traits in Maize
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Creator |
Sharma, Preeti
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Contributor |
Punia, M.S.
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Subject |
Gene effects, Generation mean analysis, maydis leaf blught, SSR markers and combining ability
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Description |
The present investigation comprised of two experiments. The first experimental material consisted of six generations i.e. parents (P1 and P2), F1, F2 and back crosses (BC1 and BC2) of six crosses namely, HKI 209 x HKI 1128,HKI 209 x HKI 163, HKI 1332 x HKI 1128, HKI 1332 x HKI 163, HKI 325-17AN x HKI 1128, HKI 325-17AN xHKI 163 to study the inheritance of maydis leaf bligh resistance and genetics of quantitative traits including yield and quality characters as well as to investigate the association of maydis leaf blight resistance with molecular markers. The second experiment was carried out in Line x Tester mating design with 9 normal and productive inbred lines viz., HKI 1128, HKI 288-2, HKI 659-3, HKI 323, HKI 1126, HKI 1105, HKI 536YN, HKI 488, HKI 1040-4 and 7 QPM testers viz., HKI 163, HKI 161, HKI 170(1+2), HKI 194-6, HKI 193-1, HKI 193-2, HKI 5072-BT(1-2)-2 with two standard checks namely, HQPM 1 and HM 5, conducted to study the GCA and SCA effects of parents and crosses, respectively along with heterosis of the resulted hybrids for the chatacters viz., days to 50 % tasseling, days to 50 % silking, days to maturity, plant height (cm), ear height (cm), no. of cobs per plant, cob length (cm), cob diameter (cm), number of grains per cob, 100 grain weight (g), grain yield per plant (g), shelling percentage, protein content (%), lysine content (%), tryptophan content (%), oil content (%), starch content (%). Both the experiments were conducted in the Department of Genetics and Plant Breeding, CCS Haryana Agricultural University (CCSHAU), at experimental area of CCSHAU, Regional research station, Uchani, Karnal, India, during two successive years of 2013-14 and 2014-15. The results have shown that inheritance of maydis leaf blight resistance was polygenic or quantitative in nature with predominance of the additive effects which was also confirmed by SSR marker analysis. It has been observed that there was a strong association between field results for inheritance of maydis leaf blight and SSR marker studied. Generation mean analysis revealed that additive-dominance model was fit for shelling percentage and oil content. Both additive[d] and dominance [h] gene effects were significant for most of the traits studied along with significant epistatic gene interaction (i, j and 1) were also observed for almost all the traits. However, the magnitude and direction of estimates varied from cross to cross. Duplicate type of interaction was apparent for all the traits. Complementary type of interaction was also recorded for for cob length, cob diameter, shelling %, protein content and oil content. The GCA effects indicated that among females, HKI 323 was good general combiner for early flowering and maturity as well as for short plant height, ear height, number of grains per cob, grain yield per plant and oil content; HKI 536YN was best combiner for lysine and tryptophan content, short plant height and number of grains per cob; HKI 536YN was best combiner for lysine and tryptophan content, short plant height and number of grains per cob; HKI 1040-4 was good combiner for phonological as well as yield and quality traits. Among the males, HKI 163 was found to be a good general combiner for grain yield per plant and quality traits studied; HKI 161 was best combiner for grain yield per plant and lysine content. On the basis of SCA effects, as well as heterotic effects 5 crosses namely, HKI 1128 x HKI 163 for yield and yield contributing traits; HKI 1126 x HKI 161 for phonological and yield traits; HKI 488 x HKI 170(1+2) and HKI 659-3 x HKI 193-2 for quality traits and HKI 1040-4 x HKI 163 for both yield and quality traits were promising. Significant heterosis was observed for all the traits. SCA variances were higher than GCA variances which indicated that one can go for hybrid breeding programme. In both the experiments, grain yield per plant showed significant positive correlation with days to 50 % teaselling, days to 50 % silking, days to maturity, plant height, ear height, cob length, cob diameter, number of grains per cob, 100 grain weight, shelling percentage, lysine content yield contributing traits whereas it was found significant negative with protein and starch content. |
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Date |
2016-08-19T14:21:41Z
2016-08-19T14:21:41Z 2016 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/73042
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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