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EVALUATION OF PHASE-RELATED MUTUALISM AND INSECTICIDAL ACTIVITY OF PHOTORHABDUS LUMINESCENS, REQUIRED BY ITS NEMATODE HOST HETERORHABDITIS INDICA

KrishiKosh

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Title EVALUATION OF PHASE-RELATED MUTUALISM AND INSECTICIDAL ACTIVITY OF PHOTORHABDUS LUMINESCENS, REQUIRED BY ITS NEMATODE HOST HETERORHABDITIS INDICA
M.Sc.
 
Creator KOMMU KIRAN KUMAR
 
Contributor Sharad Mohan
 
Subject bacteria, biological phenomena, application methods, biological development, eggs, developmental stages, animal developmental stages, nutrients, sexual reproduction, polysaccharides
 
Description The production of H. indica raised either on P or M form Photorhabdus cells was studied with an in vitro system. The bacteria proliferated in the media to provide food source for the sterile nematode eggs to replicate. Though, initially the moulting was slow in plates supplemented with M form bacteria, the growth rate of the first generation J1, J2, J3, J4, males and second generation females was statistically at par with the P form cells. Significant difference was observed in the development of hermaphrodites, followed by the subsequent second generation of J1, J2, J3 and J4 stages between 10 to 30 days. The hermaphrodites developed in P form plates were larger in size and produced more number of eggs than those recovered in M form plates. Both, amphimictic and automictic reproduction occurred in the second generation, in both the treatments, between 25 and 35 days. Endotokia matricida was also a common feature in both nematode generations. In the control plates without any bacterial supplement, the eggs hatched to J1 and could not develop beyond J2 stage even till 35 days. Pathogenicity of P, M and Phase II form variants of Photorhabdus was tested by injecting 1, 10, 100, 500 and 1000 cells/ 4th instar G. mellonella. Though mortality was relatively faster in P form cells as compared to M form, with respect to all the 5 doses tested, there was no statistical difference between the two. Higher bacterial doses of 100, 500 and 1000 cells/ larva were significantly at par in their mortality pattern, whereas, the lower two doses of 1 and 10 cells/ larva were found to be significantly different. Injection with 1 cell/ larva resulted in a maximum of 40% mortality after 42h in both the forms. In comparison to P and M form variants, the P II cell variants resulted in significantly poor mortality.
 
Date 2016-03-21T18:25:31Z
2016-03-21T18:25:31Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65347
 
Language en_US
 
Format application/pdf
 
Publisher IARI, DIVISION OF NEMATOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI