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EFFICACY OF SILICON UNDER WATER DEFICIT STRESS IN WHEAT: BIOCHEMICAL AND PHYSIOLOGICAL PERSPECTIVES

KrishiKosh

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Title EFFICACY OF SILICON UNDER WATER DEFICIT STRESS IN WHEAT: BIOCHEMICAL AND PHYSIOLOGICAL PERSPECTIVES
 
Creator SAPRE SARANG SUDHIR
 
Contributor Vakharia D.N.
 
Subject EFFICACY OF SILICON UNDER WATER DEFICIT STRESS IN WHEAT: BIOCHEMICAL AND PHYSIOLOGICAL PERSPECTIVES
BIOCHEMISTRY
 
Description Key words: antioxidative enzymes, compatible solutes, foliar silicon, MSI, oxidative stress factors, RWC, water deficit stress, wheat, 2D-PAGE
Wheat is the staple food in Western India (Saurashtra), which is prone to drought due to irregular and erratic monsoon. Silicon is a beneficial element, whose role with respect to abiotic stress is unclear. Therefore, the experiment was conducted to understand the role of silicon in abiotic stress.
The experiment was conducted in two parts. In the first experiment, the effect of silicon as an ameliorant against drought induced by polyethylene glycol- 6000 (PEG) in different varieties of wheat at the seedling stage was examined, where the treatments consisted of T1- control, T2- 20% PEG, T3- 1.5mM SiO2; T4- 1.5mM SiO2 + 20% PEG. From this one tolerant and one susceptible variety was used for protein characterisation using 2D-PAGE. In second part of the experiment, the role of silicon treatment against water deficit stress (drought) was assessed in pots and the foliar spray of silicon was applied at 15th and 30th DAG. At booting, flowering and grain filling stages, the following treatments T1- control, T2- 1.5mM SiO2, T3- water deficit stress by withholding water, T4- 1.5mM SiO2 + water deficit stress were given.
Foliar silicon (SiO2) treatment was given at 7th and 14th DAG to ten different varieties of wheat (Triticum aestivum L.) against polyethylene glycol-6000 (PEG) induced stress on 15 days old seedling for 24 hours. SiO2 treatment was able to maintain the water status in the seedlings. Application of silicon under water deficit conditions gave rise to a 17.69% increment in relative water content (RWC) as compared to PEG stress and improved the membrane stability index (MSI) under normal and PEG stress conditions. There was a decreased rate of antioxidative enzyme activity in stressed seedlings as compared with the control whereas, the silicon treatment led to a rise in antioxidative enzyme level in most cases. Proline increased under water deficit conditions, but in contrast to this SiO2 application led to a decrease in the content. The silicon content was reduced upon the imposition of stress in most of the varieties. Significant positive correlations were found between enzymatic antioxidants, RWC and MSI. Malondialdehyde (MDA) and proline displayed a significant positive correlation.
In the susceptible variety (GW 496), the maximum spots were found in SiO2 + PEG treatment in 2D-PAGE, while in the tolerant variety (GW 366) it was true for SiO2 treatment. Overall tolerant variety showed more number of spots as compared to the susceptible one. There were qualitative and quantitative differences amongst the different treatment combinations for each of the variety.
In pot study, water deficit led to a significant reduction in the RWC. As the stages progressed the per cent decline was higher in drought treatment as compared to silicon + drought treatment. The MSI significantly declined under water deficit stress, with the progression of plant age and in the susceptible variety. The chlorophyll content and chlorophyll stability index was higher in the resistant variety. The chlorophyll content showed negative correlation (P
 
Date 2016-09-28T17:35:03Z
2016-09-28T17:35:03Z
2015-01
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/79391
 
Language en
 
Format application/pdf