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Biometrical and transformation studies in tomato (Solamum lycopersicum L.)

KrishiKosh

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Title Biometrical and transformation studies in tomato (Solamum lycopersicum L.)
 
Creator R.M.Hosamani
 
Contributor A.A.Patil
 
Subject Horticulture
 
Description Biometrical and transformation studies in tomato (Solanum lycopersicum L.) were
conducted at the University of Agricultural Sciences, Dharwad, India during 2007-2010 to
find high yielding genotypes, F1 hybrids and to develop genetic transformation. Variability,
heritability, genetic advance, correlation, path analysis, genetic divergence, stability
parameters were studied from evaluation of 41 tomato genotypes during kharif 2007, kharif
2008 and rabi 2008. Heterosis and combining ability was estimated from evaluating 25 F1’s
developed in line x tester method during rabi 2008. Genetic transformants (T0) with ‘TRP
sas/ihp’ gene construct to develop resistance against tomato leaf curl virus disease in ‘DMT-
2’variety were identified.
Highly significant variation was observed amongst themselves for all the 19
characters in all seasons. High GCV, PCV, heritability and GAM were observed for plant
height, fruits per cluster, fruits per plant, locules per fruit, TSS, fruit length and width in all
the seasons. Yield had significant positive association with number of fruits per plant, TSS
and number of fruits per cluster. Yield was directly affected mainly by single fruit weight and
number of fruits per plant in positive direction.
D2 analysis indicated that number of fruits per plant, single fruit weight, plant height,
fruit length were major contributors to divergence over seasons. Genotypes were grouped
into six, five and nine clusters in three seasons.
Stability analysis showed that ‘HADT-294’, ‘PAU-2371’, ‘Dwd-T-1’ and ‘Dwd-T-6’
were well adapted to all the environments. ‘PAU-2372’, ‘VR-35’, ‘HADT-294’ and ‘ALT-
02-39’ were higher yielders per hectare. ‘VR-35’ had high yield per plant and higher single
fruit weight.
The average heterosis in nineteen characters varied from -3.28 to 18.65 per cent. It
was 15.86 per cent for yield per plant. Single fruit weight had highest gca and sca effect
values. ‘DVRT-2’, ‘H-24’, ‘Megha (L-15)’, ‘Dwd-T-3’, and ‘Dwd-T-6’ were overall high
general combiners. Among the F1’s, DVRT-2 x VR-35 (48.47 t), Pant-T-10 x VR-35 (45.65
t), DVRT-2 x KS-227 (45.09 t) and DVRT-2 x Dwd-T-6 (43.71 t) were top yielders per
hectare and incidentally yield per plant too was higher in these hybrids.
The transgenic ‘DMT-2’ tomato plants (T0) were obtained through Agrobacterium
mediated transformation with ‘TRP sas/ihp’ gene construct for silencing the transcription of
ToLCV gene encoding replicase. The transgene integration in plant genome was confirmed
throug h PCR amplification of hpt II gene.
 
Date 2016-07-23T11:26:41Z
2016-07-23T11:26:41Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69512
 
Format application/pdf
 
Publisher UAS Dharwad