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STUDIES ON VITRIFICATION OF ENTOMOPATHOGENIC AND PLANT PARASITIC NEMATODES

KrishiKosh

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Title STUDIES ON VITRIFICATION OF ENTOMOPATHOGENIC AND PLANT PARASITIC NEMATODES
M.Sc.
 
Creator BHUPAL NAMDEO HATZADE
 
Contributor Sharad Mohan
 
Subject alcohols, biological phenomena, nitrogen, cryopreservation, carbohydrates, diseases, cryoprotectants, ethylene, eggs, storage
 
Description Out of eight cryoprotectants viz. ethylene glycol (10 and 20%), glycerol (15
and 20%), dimethyl sulfoxide (10 and 20%), glycerol (15%) + methanol (70%) and
glycerol (20%) + methanol (70%) tested for cryopreservation, for a period of one
week, 10% ethylene glycol for M. incognita and 20% ethylene glycol for H. indica
were found to be the most efficient in providing 14.43 and 11.25% survival,
respectively. It was followed by 20% DMSO in providing 11.15% survival of H.
indica and 14.13% survival of M. incognita. Uniformly, in all the treatments the dead
juveniles of M. incognita and H. indica were found lying straight and in a few cases
they found to be ruptured after removing from liquid nitrogen. The cryo infective
juveniles of M. incognita and H. indica recovered after 14, 28 and 56 days of
preservation in liquid nitrogen did not lose their infectivity and were able to penetrate
and reproduce on tomato seedlings and Galleria larvae, respectively. Effect of
vitrification of M. incognita eggs did not provide any encouraging results. After
thawing only 0.43, 0.50 and 0.40 % eggs hatched in 10 % ethylene glycol, 20 %
glycerol and 20 % DMSO, respectively, as compared to 51.82% hatching observed in
untreated control.
 
Date 2016-04-30T12:22:36Z
2016-04-30T12:22:36Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65683
 
Relation t-8853;
 
Format application/pdf
 
Publisher IARI, DIVISION OF NEMATOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI