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VARIATION IN WHEAT GENOTYPES FOR SPORE LOAD OF Bipolaris sorokiniana

KrishiKosh

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Title VARIATION IN WHEAT GENOTYPES FOR SPORE LOAD OF Bipolaris sorokiniana
 
Creator Satyaswara Jasudasu Gompa
 
Contributor Prof. Ramesh Chand
 
Subject Wheat, fungal disease Bipolaris sorokiniana, Isolation and morphological characterization and SSR markers
 
Description M. Sc. (Ag.)
The experiment was carried out at the Agricultural Research Form, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi during rabi season of 2012-2013. The experiment was laid out in randomized block design with two replications. Observations were recorded on leaf angle, leaf tip necrosis and days to heading. Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat in warm and humid wheat growing regions of the world. The present study was under taken using 294 WAM genotypes with the objective to assess the seed transmission of B. sorokiniana in different wheat growing regions. Total 40 genotypes representing the out of 294 genotypes for spore load/ 50 seeds with the objectives to select an easy and precise method. Correlation between two spore counting methods studied. The correlation of spore load, with the characters influence spot blotch i.e. leaftip necrosis, leaf angle and days to heading resistance markers were established. The following conclusions drawn from the present study: The region Varanasi has highest spore load as compared to the Karnal (Haryana) and Coochbehar (West Bengal). It means high incidence of spot blotch in this region. The Karnal has lowest spore count and indicated the region has very low spot blotch incidence. Among the two spore counting methods (i.e Nematode Plate count Method and Washing Test method) a correlation coefficient 0.8 was established. The paired t- test results indicated that the Nematode Plate count Method was superior to the Washing Test method. The mean recovery of spore in Nematode Plate Count Method was higher as compared with the Washing Test method. Negative but significant correlation was observed between Days to Heading and spore load of the both the methods. It means increasing days to heading would reduce the spore load. SSR marker Xgwm111 and Xgwm148 linked with the QTLs for spot blotch resistance QSb.bhu-7D and QSb.bhu-2B were amplified corresponding resistant and susceptible bands in most of the resistant and susceptible genotypes. Therefore these markers could be used in MAS for spot blotch resistance in wheat. The clustering of all the genotypes was according to their reaction to spot blotch.
 
Date 2016-09-13T13:17:52Z
2016-09-13T13:17:52Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76494
 
Language en
 
Relation M0021;
 
Format application/pdf
 
Publisher Institute of Agricultural Sciences, Banaras Hindu University