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DEVELOPMENT OF FUNCTIONAL MARKER FOR GRAIN WEIGHT GENE GW2 IN RICE

KrishiKosh

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Title DEVELOPMENT OF FUNCTIONAL MARKER FOR GRAIN WEIGHT GENE GW2 IN RICE
 
Creator VENKAT REDDY, P
 
Contributor SHESHU MADHAV, M
 
Subject grain, rice, genes, alleles, genetics, pcr, selection, breeds (animals), dna, planting
 
Description Rice is central to the lives of billions of people around the world. Rice is vital to
global food security, being staple food for more than 60 % of world’s population. Rice
is an excellent model system for genome studies and evolution of crop plants among
monocot species, because of its small genome size and the availability of high-density
molecular marker linkage maps. Significant progress in rice functional genomics is
being made since the completion of the international rice genome sequencing project.
With an ever-increasing global population but rapid shrinking of fertile cultivable
lands day by day and declining yield stability in cereals is one of the major issues, the
India is facing at present. There is an urgent need to maintain the yield stability of rice
and further improve the rice grain yield using molecular genetic breeding approaches.
Changes in a desirable variety can be achieved more rapidly through molecular
breeding than with traditional breeding techniques. The presence of the desired gene
controlling the trait can be tested for at any stage of growth, such as in small seedlings
in a greenhouse tray. The precision and versatility of today’s biotechnology enable
improvements in food quality and production to take place more rapidly than when
using traditional breeding.
In traditional breeding, crosses are made in a relatively uncontrolled manner. The
breeder chooses the parents to cross, but at the genetic level, the results are
unpredictable. DNA from the parents recombines randomly, and desirable traits are
bundled with undesirable traits, such as lower yield or poor quality.
The present investigation is carried out with the objective of development of
functional marker for grain weight (yield component). Of the three reported SNPs (Song
et al. 2007), an SNP at 114 nucleotide position (C/T) was targeted and primers were
designed and standardized at an annealing temperature of 64oC. Validation was carried
out in the total selected germplasm (124varieties) and observed the dominance of low
grain weight allele “C”. The high grain weight allele “T” was present very rarely
indicating that, it may have arisen recently from the process of rice domestication.
Since the marker (namely DRR-GW) showed very less association with the grain
weight, an attempt was made to know the association between kernel length and grain
weight, which showed a good association. Hence, it may be illated that, as the newly
developed marker DRR-GW was targeted only one functional polymorphism i.e. SNP at
114 nucleotide position of GW2 which may not be sufficient enough to explain the
variation exist in the grain weight phenotype of selected germplasm.
 
Date 2016-06-23T09:19:28Z
2016-06-23T09:19:28Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67769
 
Language en
 
Relation D9138;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY