Record Details

Studies on physiological and biochemical aspects of seed priming in onion (Allium cepa L.)

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Studies on physiological and biochemical aspects of seed priming in onion (Allium cepa L.)
 
Creator Khanduri, Shruti
 
Contributor Kharb, R.P.S.
 
Subject Sowing, Storage, Enzymes, Developmental stages, Germinability, Proteins, Seed treatment, Irrigation, Solutes, Onions
 
Description The present investigation was carried out at the department of Seed Science and Technology,
CCS Haryana Agricultural University, Hisar. The seed material comprised of two lots (L1 and L2) of
each of two onion varieties (Hisar-2 and Hisar Onion-3). The seed of each variety lot combination was
treated with eight priming treatments (T1 to T8) and control (T0) was kept for comparison. The treated
seed after shade drying was stored in polythene bags (700 guage) for one year under ambient storage
conditions. The observations were recorded quarterly on various physiological and biochemical
parameters. The relative storability was assessed by ‘AA’ and EC tests.
The results revealed that among various priming treatments, the treatments T2 (GA3, 50 ppm),
T3 (GA3, 100 ppm) and T7 (Azotobacter) were found most effective and enhanced the germination
(upto 21.33%) and total seedling emergence (upto 27.00%) substantially over control in marginal seed
lots of both the varieties. However, the treatment T5 (0.5% KNO3) reduced the germination and
seedling emergence potential considerably over control. Hence, this treatments can not be suggested for
priming the onion seed.
Among the biochemical parameters the activities of catalasee and dehydrogenase enzyme
substantially increased over control after priming. These enzymes played a crucial role in metabolic
process for enhancement of germination and seedling emergence potential. Significant association of all
the parameters studied except peroxidase enzyme with total seedling emergence showed that these
parameters can be used as predictors of seedling establishment after priming. The priming treatments
can be categorized in to three groups i.e. Group I (fully effective), Group II (partially effective) Group
III (non effective).
A decreasing trend in germination and vigour level was observed in all the treatment at
different stages of storage. Among priming treatments T2 & T3 (seeds treated with gibberellin, GA3)
were also found most effective for enhancing the storability of different seed lots as these treatments
maintained the highest germination successfully (above 60%) after accelerated ageing and recorded EC
values below 0.4 S/cm till the end of the storage. There was a very little change in protein
macromolecules was noticed, however, no change was observed in banding pattern of different
isozymes (CAT, POD, SOD) even after twelve months of storage. The priming technology was found
effective and beneficial for enhancing the quality of onion seed.
 
Date 2016-11-10T09:55:01Z
2016-11-10T09:55:01Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/84791
 
Language en
 
Format application/pdf
 
Publisher CCSHAU