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ESTIMATION OF OLIGOSACCHARIDES CONTENT IN LOCALLY AVAILABLE FRUITS AND VEGETABLES

KrishiKosh

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Field Value
 
Title ESTIMATION OF OLIGOSACCHARIDES CONTENT IN LOCALLY AVAILABLE FRUITS AND VEGETABLES
 
Creator SIDDIRAM SIDDESHWAR
 
Contributor KONDAL REDDY, K
 
Subject ESTIMATION, OLIGOSACCHARIDES, CONTENT, LOCALLY, AVAILABLE, FRUITS, VEGETABLES
 
Description An attempt was made in the present study to estimate the oligosaccharide
content in fruits, vegetables and roots and to find the effect of drying on different
oligosaccharides (stachyose, raffinose, maltotriose, maltotetraose, maltopentaose,
maltohexaose and maltoheptaose) considering their importance as prebiotics.
Quantification, involved hot water extraction (98 0C/ 2 hrs) of oligosaccharides,
initial filtration (soy cloth), secondary filtration (ordinary filter paper), tertiary
filtration (whatman No.42 paper) and final filtration ( membrane 0.25μ) followed by
high performance liquid chromatography (HPLC) analysis with 70 per cent
acetonitrile as eluent, using refractive index (RI) detector and oligosaccharide
standards as reference. Repetitive injection of different concentrations of standard
sugars was done to check the detector response.
Forty two different samples extracted from fruits, vegetables and roots were
analyzed for the presence of oligosaccharides mentioned. Mainly raffinose content
was observed (mg/ g of dry matter) in ladies finger (0.089), onion (0.3015) and
potato in trace amounts.
Stachyose content was noticed (in mg/ g of dry matter) in apple (traces only)
Maltotriose content was observed (mg/ g of dry matter) in onion (0.1148).
Maltotetraose content was observed (mg/ g of dry matter) in beet root (0.2394),
ladies finger (traces), onion (0.105), garlic (0.3075), guava (0.0678) and banana
(0.177).
Maltopentaose content was found in three samples (mg/ g of dry matter) in
yam (traces), garlic (0.3929) and tomato (0.549). Maltohexaose content was present
(mg/ g of dry matter) in carrot but only in traces. Maltoheptaose content was not
found in any analyzed samples (fruits, vegetables and roots).
In few samples, more than one oligosaccharides were observed such as in
ladies finger (raffinose, maltotetraose), onion (raffinose, maltotriose and
maltotetraose) and garlic (maltotetraose, maltopentaose).
In samples like radish, corm, pumpkin, mango, sapota, papaya, grapes,
pineapple and bael fruit, oligosaccharides were not found for which analysis was
made.
All those oligosaccharides found in fresh samples were also observed in
dried samples and were proportionate to the dry matter indicating that drying had no
effect on enzymatic hydrolysis of complex starches to produce higher levels or
different oligosaccharides in dried samples compared to fresh samples.
Carrot, Ladies finger, onion, garlic, beet root, yam, guava, apple, tomato and
banana appear to be good sources for commercial extraction of prebiotics by using
membrane processing technology.
 
Date 2016-08-20T14:06:38Z
2016-08-20T14:06:38Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73179
 
Language en
 
Relation D7685;
 
Format application/pdf
 
Publisher ACHARYA N.G.RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD