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MAPPING OF YELLOW MOSAIC VIRUS (YMV) DISEASE RESISTANCE IN SOYBEAN [Glycine max (L). MERR.]

KrishiKosh

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Title MAPPING OF YELLOW MOSAIC VIRUS (YMV) DISEASE RESISTANCE IN SOYBEAN [Glycine max (L). MERR.]
Ph.D.
 
Creator HARISH.G.D
 
Contributor Talukdar, Akshay
 
Subject soybeans, diseases, planting, genotypes, genes, genetics, polymorphism, land resources, dna, biological phenomena
 
Description Soybean is the numero uno oilseed crop in India. It is primarily used as source of oil and protein. Production and productivity of soybean is challenged by a number of biotic and abiotic factors. Yellow mosaic virus (YMV) disease caused by begomovirus and transmitted by whitefly (Bemisia tabaci) is an important biotic challenge. With an aim to identify sources of resistance, a set of 100 germplasm lines was screened in the field during kharif 2009, 2010 and 2011. Twenty six (26) genotypes were identified that exhibited resistance consistently for 3 years. Using PCR-based markers, the virus strain causing YMV disease in IARI, New Delhi was identified to be ‘mungbean yellow mosaic India virus’ (MYMIV). The strain-specific markers could effectively identify the cases of ‘disease escape’ and ‘true resistance’. Field testing of F1 and F2 populations of three cross-combinations viz., PI542044 x DS9712, PI542044 x DS9814 and JS335 x DS9712 indicated that resistance to MYMIV strain is caused by a single, dominant gene. The gene was tentatively designated as Ymv-IV (IV for India virus) and mapped to the short arm of chromosome No. 17 (Linkage group D2s). Of the 170 SSR markers tested, only 80 were polymorphic (47.06% polymorphism). Bulked segregants analysis (BSA) identified two markers viz., Sat_326 and Satt528 to be linked to Ymv-IV. Mapping analysis in 205 F2 plants with MAPMAKER/Exp v3.0b software mapped Sat_326 and Satt528 on 7.6 cM and 9.9 cM, respectively, on either side of the gene Ymv-IV. Efficacy of the markers was validated on a set of germplasm lines and a segregating population. The markers would pave the way for effective marker-assisted selection (MAS) for YMV disease resistance in soybean.
 
Date 2016-03-08T15:47:02Z
2016-03-08T15:47:02Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64941
 
Language en_US
 
Format application/pdf
 
Publisher IARI, Division of Genetics, New Delh