Record Details

EVALUATION OF CARROT GRASS(PARTHENIUM SP.) FOR ETHANOL PRODUCTION

KrishiKosh

View Archive Info
 
 
Field Value
 
Title EVALUATION OF CARROT GRASS(PARTHENIUM SP.) FOR ETHANOL PRODUCTION
Ph.D.
 
Creator PANDIYAN, , K..
 
Contributor Lata
 
Subject enzymes, cellulose, biomass, alcohols, productivity, fungi, sugar, aromatic compounds, fermentation, yields
 
Description Evaluation of carrot grass (Parthenium sp.) for ethanol production
The production of bioethanol from lignocellulosic biomass, also called second generation biofuel,provides several energetic, economic and environmental advantages over conventional sources such as sugars or starch. Amongst different type of lignocellulosic substrate, weedy biomass Parthenium sp. as a feedstock for bioethanol production is less investigated. The present investigation explores its potential for bioethanol production by using chemical and biological pretreatment methods. Acid and alkali pretreatments were compared with biological pretreatment with lignolytic fungus Marasmielluspalmivorus PK-27 isolated from decaying biomass. Structural and chemical changes were examined using scanning electron microsope (SEM), fourier transform infra red (FTIR) and X-ray diffraction (XRD) analysis after chemical and biological pretreatment. Among the three pretreatment methods, alkali (1% NaOH) treatment showed significantly higher recovery of total reducing sugars (513 ± 41.0 mggds-1) after 48 h of enzymatic hydrolysis with saccharification yield of 76.6% andrecovery of lignin as acid precipitable polymeric lignin (APPL) (7.53 ± 0.5 mggds-1). Optimization of enzymatic hydrolysis variables such as temperature, pH, enzyme loading and substrate loading rate was carried out using central composite design (CCD) in response surface methodology (RSM) to achieve the maximum saccharification yield. The experimental value of saccharification yield (85.80%) obtained was higher than the predicted value (80.08%) proposed by the developed model under optimum conditions(temperature - 50°C, pH - 4.53, enzyme loading - 8% and substrate loading - 2.4%). This illustrated a good agreement between predicted and experimental response in turns of saccharification yield.Monoculture fermentation of enzymatic hydrolysate with Saccharomyces cerevisiae LN1 resulted in ethanol yield of 0.27 g/g within 36 h while Pichiastipitis NCIM3498 produced 0.23 g/g of ethanol yield after 72 h. The co-culture fermentation with both yeasts enhanced the ethanol concentration (14.28 ± 0.31 g/L) as well as ethanol yield (0.30 g/g). These findings reveal the promise of Parthenium sp. biomass as anattractivefeedstock for bioethanol production.
 
Date 2016-03-18T16:29:20Z
2016-03-18T16:29:20Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65268
 
Language en_US
 
Format application/pdf
 
Publisher IARI, DIVISION OF MICROBIOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI