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MARKER ASSISTED PYRAMIDING OF BLAST RESISTANCE GENES IN BASMATI RICE
KrishiKosh
View Archive Info| Field | Value | |
| Title |
MARKER ASSISTED PYRAMIDING OF BLAST RESISTANCE GENES IN BASMATI RICE
Ph.D. |
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| Creator |
Shikari, Asif Bashir
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| Contributor |
A. K. Singh
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| Subject |
genes, rice, planting, selection, diseases, genetics, alleles, germplasm, backcrossing, grain
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| Description |
Basmati rice is a heritage of our country and a valuable export commodity, which earns an annual foreign exchange of Rs. 19391 crores of which nearly 70 % is contributed by two rice varieties, Pusa Basmati 1 (PB 1) and Pusa Basmati 1121. Pusa Basmati 1 in particular is highly susceptible to several diseases among which, rice blast caused by Magnaporthe oryzae is a major production constraint. Therefore, the present study was undertaken with the objective to incorporate three major genes namely, Pi54, Pi1 and Pita in the genetic background of PB 1. A non-Basmati parent, DHMAS70Q 164-2a harbouring these three genes was used as donor. Marker assisted backcross breeding was followed to develop pyramided and isogenic lines in PB 1 background.. The backcross progenies in BC2Fn, BC3Fn and BC4Fn were evaluated for agro-morphological traits, grain and cooking quality. Plants with superior phenotypic performance and desirable grain and cooking quality were subjected to marker assisted foreground selection using STMS markers RM206, RM224 and RM247 linked to Pi54, Pi1 and Pita, respectively, followed by recombinant and background selection with 27 and 58 polymorphic SSR markers, respectively. Each of the three backcross generations were pursued till BC2F6, BC3F5 and BC4F4 and plants homozygous for the genes Pi54+Pi1+Pita, Pi54+Pi1, Pi54+Pita, Pi1+Pita, Pi54, Pi1 and Pita were recovered. The extent of recurrent parent genome recovery in three-gene pyramided lines, Pusa-1633-2-1-8-3-35-91-170-6 and Pusa 1633-2-1-6-1-217-3-162-2, was 98.6 % in BC4F4 and BC3F5 generations, respectively. The pyramided lines were inoculated with specific isolates from Basmati growing belts of the country, under controlled conditions. The derived NILs and pyramided lines expressed resistance reaction to isolates and possessed excellent grain and cooking quality besides agronomic performance, at par with check PB 1, in replicated yield trial. The deployment of pyramided lines will help to achieve broad spectrum and durable resistance to rice blast in Basmati growing regions and shall prove as effective measure to safeguard the yield potential of this important trade commodity. Molecular analysis was carried out for 12 blast resistance genes in 100 germplasm accessions and the resistance specific alleles were found in 27, 27, 35, 67, 25, 8 and 28 lines for genes namely, Pita, Pi54, Pikm, Pik, Pib, Pi9 and Piz5, respectively. Phenotypic validation of germplasm and the markers was done by using four diagnostic M. oryzae isolates. Based on phenotypic and molecular analysis, the genes Piz5, Pita and Pik were found predominant in the germplasm screened under present study. Molecular markers linked to genes Piz5, Pita, Pik, Pikm, were efficient in detecting resistance specific alleles for blast resistance among a set of germplasm. Key words: Basmati, Rice, Blast disease, Gene, Pyramiding, Foreground, Background selection, Germplasm, Validation, Markers |
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| Date |
2016-03-11T13:29:21Z
2016-03-11T13:29:21Z 2013 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/65086
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| Language |
en_US
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| Format |
application/pdf
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| Publisher |
IARI, DIVISION OF GENETICS,NEW DELHI
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