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Molecular detection of Newcastle disease virus using Flinders Tehnology Associates-PCR

KrishiKosh

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Title Molecular detection of Newcastle disease virus using Flinders Tehnology Associates-PCR
 
Creator Narayanan, Madaboosi S.
Parthiban, Manoharan
Sathiya, Padmanabhan
Kumanan, K
 
Subject Newcastle disease virus
Filter paper
Molecular detection
 
Description The feasibility of using Flinders Technology Associates (FTA) fifi lter papers to store the Newcastle disease virus (NDV), infected allantoic flfl uid (AF) and tissue samples, for the molecular detection of NDV by reverse transcriptase - polymerase chain reaction (RT-PCR) - was investigated. An FTA card is a cotton based cellulose membrane, with lyophilized chemicals that lyse the viruses and bacteria. The viral RNA was detectable from FTA cards up to a concentration of 10^7.6 EID50/100 μL (a 100 times dilution of 10^9.6 EID50/100 μL of initial stock). The inactivated virus remained stable on the cards for up to 30 days, both at room temperature and 4ºC. NDV was detected by RT-PCR from all the FTA imprints of the caecal tonsils, kidney, proventriculus, spleen,
trachea, faecal swabs and intestinal lesions of NDV-suspected birds. NDV was inactivated upon contact with FTA, as shown by the inability of the virus to propagate in embryonated eggs and its inability to infect chicken embryo fifi broblast culture. In conclusion, FTA cards are suitable for collecting and transporting NDV infected
samples, without cold storage. The virus inactivated in FTA cards, however, is a suitable source of viral RNA for molecular detection and characterization.
 
Date 2016-06-22T14:28:05Z
2016-06-22T14:28:05Z
2010
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67726
 
Language en
 
Format application/pdf
 
Publisher Veterinarski Arhiv