Record Details

BULBLET REGENERATION FROM ANTHER EXPLANTS IN HYBRID LILY

KrishiKosh

View Archive Info
 
 
Field Value
 
Title BULBLET REGENERATION FROM ANTHER EXPLANTS IN HYBRID LILY
 
Creator SUBHASH, CHAND
 
Contributor SHARMA, S.K.
 
Subject System does'nt support this MIME type of file application/octet-stream; charset=ISO-8859-1
hybrid lily,Bulblets regeneration,explants,sterilized explants
 
Description ABSTRACT
In the present investigation entitled “Bulblets regeneration from anther explants
in hybrid lily”, the sterilized explants (anthers) were inoculated on MS medium
supplemented with different concentrations of growth regulators. The number of days for
callus initiation decreased significantly when MS medium was supplemented with
growth regulators in case of cultivar Casa Blanca, whereas anther of other cultivars
failed to produce any callus. The minimum number of days required for callus initiation
was observed when MS medium was supplemented with 1.0 mg/l 2,4-D + 0.5 mg/l BA.
The colour of initiated callus was yellowish green with nodular type appearance. The
number of days for bulblet initiation decreased significantly when MS medium was
supplemented with growth regulators. The maximum number of bulblets were observed
on MS medium supplemented with 1.0 mg/l NAA + 1.0 mg/l BA. The highest average
fresh weight per bulblet obtained when MS medium was supplemented with 2.0 mg/l
NAA + 1.5 mg/l BA. The maximum per cent bulblet (83.33%) forming root and
maximum roots per bulblet (4.67) was observed with 2.0 mg/l IBA. The highest root
length (16.00 mm) was observed on 1.5 mg/l NAA. Out of 17 regenerated plantlets 8
plantlets showed variation in chromosome number ranging from 20-26, while other
plantlets had normal chromosome number i.e., 2n=24.
 
Date 2017-01-04T12:24:12Z
2017-01-04T12:24:12Z
2012
 
Type Thesis
 
Identifier 47721
http://krishikosh.egranth.ac.in/handle/1/94389
 
Language en
 
Format application/pdf