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Immunoprophylaxis Against Haemonchus Contortus In Sheep Using Excretory / Secretory Antigens

KrishiKosh

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Title Immunoprophylaxis Against Haemonchus Contortus In Sheep Using Excretory / Secretory Antigens
 
Creator Arunkumar, S
 
Contributor Basith, S. Abdul
Gomathinayagam, S
Chandran, Daniel Joy
Raj, G. Dhinakar
 
Subject Sheep
Haemonchus contortus
Excretory / Secretory antigens
Immune response
Cross reaction
 
Description Excretory / secretory (E/S) antigen from Haemonchus contortus
was collected by culturing live adult worms in RPMI 1640 medium at 37 o C for 24
hours and the culture supernatant was used as antigen. Thiol-sepharose affinity
chromatography was used for the purification of E/S antigen.
On immuno blot analysis, it was observed that the total E/S antigen
showed five reactive bands at 24, 29, 46, 66 and 93 kDa. A single band at
66 kDa was detected in affinity purified antigen. On cross-reactive analysis, it
was observed that there was no cross-reactivity between E/S antigens of
H. contortus and Oesophagostomum columbianum.On immunization trial, it was observed that the affinity purified
antigen elicited high antibody responses than total E/S antigen by ELISA. On
flow cytometry analysis, it was observed that the mean percentage of CD4 +
T-lymphocytes count was higher in purified antigen immunized group than total
E/S antigen immunized group. The mean percentage of CD8 + T-lymphocytes
count was significantly lower in immunized group. This study clearly revealed
that CD4 + T-lymphocytes played a significant role in inducing cellular responses
in immunized animals. The mean EPG values were significantly lower in purified
antigen group than total E/S antigen group. The percentage reduction in mean
faecal egg count was 88.5 per cent and 76.8 per cent in purified and total E/S
antigen groups, respectively.
The mean abomasal worm counts were
significantly lower in purified antigen group than total E/S antigen group. The
percentage reduction in mean abomasal worm count was 75.4 per cent and
67.12 per cent in purified and total E/S antigen groups, respectively. It was also
observed that the slaughter weight of immunized sheep was higher than the
control group.
 
Date 2016-05-17T15:29:24Z
2016-05-17T15:29:24Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66038
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University