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Studies on mass production of beauveria bassiana (Bals.) vuill., its bioefficacy and compatability with botanicals against pigeonpea pod borer complex

KrishiKosh

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Title Studies on mass production of beauveria bassiana (Bals.) vuill., its bioefficacy and compatability with botanicals against pigeonpea pod borer complex
 
Creator Jain, Deepak
 
Contributor Das, S.B.
 
Subject oils, fungi, plant extracts, biological development, bugs, planting, animal developmental stages, beauveria bassiana, productivity, pigeonpeas
ENTOMOLOGY
 
Description ABSTRACT



The present investigation entitled, “Studies on mass production of Beauveria bassiana (Bals.) Vuill.,its bioefficacy and compatibility with botanicals against pigeonpea pod borer complex’’ was carried out in the experimental field of Department of Entomology, Live Stock Farm, Adhartal, JNKVV, Jabalpur (M.P.) during Kharif 2014-15. The laboratory studies were conducted in the Entomology Laboratory, College of Agriculture, JNKVV Jabalpur.
The experiment was conducted with the following three objectives:

1. To study the influence of temperature and nutrient on mass production of

Beauveria bassiana on local substrates.

2. To study the bioefficacy and compatibility of B. bassiana with botanicals against pigeonpea pod infesting insect pest complex.

2.1. Invitro studies on compatibility of B. bassiana with botanicals.

2.2. Bioefficacy and compatibility of B. bassiana with botanicals against pigeonpea pod borer complex under field condition.
3. To study the shelf life of B. bassiana under normal conditions.

Among the substrates, highest spore production and spore viability was observed in rajma incorporated with dextrose and incubated at room temperature (245C), followed by pea and cowpea, while lowest was
observed in green gram without dextrose and at incubated 35C temperature.
The present study also supports the fact that among several naturally available substrates tested for mass multiplication of B. bassiana, PDB and okra were most economically cheap substrates. It was also clear that B. bassiana is able to grow on a variety of cheap and easily available substrate and its growth can be further enhanced by adding dextrose under normal condition (25-30C).Hence the fungus can be produced in bulk and can made
easily available to the farmers.

The effect of botanicals on the mycelial growth of B. bassiana was conducted invitro. All the treatments showed significant mycelial growth but less than control. Among the botanicals tested, neem oil 2.5% (v/v) (half recommended dose) was found to be most compatible with least growth inhibition followed by neem soap 0.5% (w/v).

Although the different botanicals tested in the present investigation, slightly inhibited the growth of B. bassiana in poisoned media invitro, the combined use of the fungus and botanicals cannot be completely ruled out. All the botanicals tested have been combined with B. bassiana for obtaining better control of pod borer complex in pigeonpea. Considering this, it is worth exploring the effect of these botanicals at sub lethal dose with fungus for an enhance result over pest control.

On the basis of the effectiveness of different treatments against pod borer complex and the grain yield obtained, it can be said that B.bassaina + neem soap (Half dose of 1x108spores/ml+0.5%(w/v)) treated plots followed B.bassiana +pongamia oil(Half dose of
1x108spores/ml+2.5%(v/v)) and B. bassiana + neem oil (Half dose of

1x108spores/ml+2.5%(v/v)) proved to be the most effective treatments not only in reducing the damage due to pod borer complex, but also recorded higher grain yields (986.88,955.93 and 900.00 kg/ha), respectively.

Maximum net profit was obtained in case of B. bassiana + neem soap (Rs.36337/-per ha) followed by B.bassiana + pongamia oil (Rs
34231/-per ha) and B.bassiana + neem oil (Rs. 31926/-per ha), respectively

Taking into account the cost of plant protection inputs, the cost benefit ratio of neem oil was highest (1:12.2) followed by B. bassiana + neem soap (1:10.7). It means that a farmer will likely get a profit of Rs. 12=20 or Rs.
10=70 after investing an amount of Rs. 1/- on plant protection measure by applying neem oil or B. bassiana + neem oil, respectively.

By decreasing the dose of botanicals and incorporating microbials as combination treatments in pest management program can give

the farmer the advantage of environmental friendly farming and higher economic returns.

The shelf life of a microbial pesticide is an important factor for insect control .In the present study aqueous formulation of B.bassiana kept in high density black and white coloured plastic bottles and stored for six months under normal condition, revealed that there was no impact of the colour of the bottle on the spore viability. Further upto 150 days of storage of B.bassiana under normal condition, there was no significant reduction in the spore viability.
 
Date 2016-06-28T14:25:29Z
2016-06-28T14:25:29Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/68153
 
Language en
 
Format application/pdf
 
Publisher JNKVV