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Detection Of Peste Des Petits Ruminant (PPR) Virus By Real Time PCR

KrishiKosh

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Title Detection Of Peste Des Petits Ruminant (PPR) Virus By Real Time PCR
 
Creator Dhok, A. K.
 
Contributor Ingle, V. C.
 
Subject Veterinary Biotechnology
Detection Of Peste Des Petits Ruminant (PPR) Virus By Real Time PCR
 
Description The present study was planned for detection of PPRV from clinical
samples using real time PCR assay.
In present study 13 clinical samples (8 blood, 3 nasal swab, 2 tissue
samples) as a representative from suspected sheep and goat were collected from Nagpur and Mouda tahsil in Nagpur district of Maharashtra.
Detection of PPRV isolates was carried out using conventional method
like RT-PCR as well as Real time PCR assay of M gene using SYBR
green method.
Conventional RT-PCR assay revealed that among these 13 clinical
samples, 6 samples (3 blood samples, 1 nasal swab and 2 tissue
samples) were found positive for PPRV indicating 46.15 % positivity.
However, Real time PCR assay of M gene using SYBR green method
revealed that all samples were positive for PPR indicating 100 %
positivity.
From the result it has been revealed that the Real time PCR of M
gene using SYBR green assay has the advantages over the conventional
RT-PCR and found to be rapid, sensitive and a suitable screening tool in
addition to the earlier available techniques for confirmatory detection of
PPR viruses in the clinical specimens of PPR suspect cases.
 
Date 2017-01-03T16:54:13Z
2017-01-03T16:54:13Z
2012-09-16
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94273
 
Language en
 
Format application/pdf