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STUDIES ON STANDARDIZATION OF TECHNIQUES FOR CALLUS INITIATION, GROWTH AND DIFFERENTIATION IN RICE (oryza sativa L.)

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Title STUDIES ON STANDARDIZATION OF TECHNIQUES FOR CALLUS INITIATION, GROWTH AND DIFFERENTIATION IN RICE (oryza sativa L.)
 
Creator PRITHIIVI RAJ, KAPARABOINA
 
Contributor SIVARAMA REDDY, C
 
Subject STUDIES, STANDARDIZATION, TECHNIQUES, CALLUS, INITIATION,GROWTH,
 
Description In the present study, an attempt was made to
standardize the cultural conditions such as type of
media, type of explant, hormonal requirements for callus
initiation, growth and morphogenesis and to determine
the differences in peroxidase isozyme pattern in
different explants, their callus, at different stages of
differentiation and in regenerated plantlets of three
different rice cultivars namely, Tellahamsa, Sonamashuri
and Tetraploid (Basmati x Tellahamsa).
Among different explants, higher frequency of
callus initiation and good callus growth was observed in
seed explants compared to that of embryo explant in all the varieties. Among different varieties, Tellahamsa
responded better for callus initiation, growth and
differentiation followed by Sonamashuri and Tetraploid.
Among different Media and hormonal combinations (2,4-D,
IAA, NAA, . KIN, BAP) tested, LS medium supplemented
with 4 mg/1 2,4-D and 0.5 mg/1 KIN was found to be good
for callus induction and growth of all types of ยท explants
in all cultivars, though callus initiation was observed
in both MS and LS media.
For morphogenesis and complete plantlet
regeneration, LS medium supplemented with 4 mg/1 KIN + 1
mg/1 IAA was found to be ideal followed by MS medium
with 4 mg/1 KIN + 1 mg/1 IAA. Higher frequency of
plantlet regeneration was observed from seed callus,
while very low frequency of plantlet regeneration was
observed from embryo derived callus in all the
var~eties. With increased concentration (2mg/l to
4mg/l) of cytokinin increased plantlet regeneration was
observed. Complete plantlet regeneration of 90%, 85%
and 60% from seed derived callus was observed in
Tellahamsa, Sonamashuri and Tetraploid, respectively.
About 50-80% of the plants survived when the regenerated
plantle~ were transferred to field after hardening and
all of them behaved normally with good flowering and
seedset.
Electrophoretic studies indicated the presence
of more number of peroxidase isozymes in different
explants of Sonamashuri (12 bands) followed bY
Tellahamsa (11 bands) and Tetraploid (7 bands). In
general, more number of peroxidase isoz'ymes were found
in embryo explant of Tellahamsa followed by Sonamashuri
and Tetraploid compared to seed explant, their callus
and regenerated plantlets.
In case of whole plants, the band with Rm value
0.34 was found specific to Tellahamsa, the band with Rm
value 0.4 was !ound specific to Sonamashuri, while the
bands with Rm values 0.43, 0.53 and 0.6 were found
specific to regenerated plantlet of Tetraploid. Certain
common bands such as the band with .Rm value 0.39 was
found common to seed callus, differentiated callus,
regenerated plantlet and embryo callus in Tellahamsa.
The band with Rm value 0.26 found common to all explants
in Sonamashuri and in tetraploid, the band with Rm value
0.48 was found common to all explants.
In general, less peroxidase isozymes were
observed in in vitro grown seedlings, when compared with
in vivo (seed) grown seedlings. The differences in total
number of bands, specific bands and common bands in
different explants and varieties observed in the present
study could used for identification of different rice
cultivars.
 
Date 2016-08-05T14:16:05Z
2016-08-05T14:16:05Z
1988
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71064
 
Language en
 
Relation D3032;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD