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ISOLATION, IDENTIFICATION, PHYSIOLOGICAL STUDIES AND EVALUATION OF POTENT ENTOMOPATHOGENIC FUNGUS Beauveria bassiana (Bals.)Vuill AGAINST Spodoptera litura (Fab).

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Title ISOLATION, IDENTIFICATION, PHYSIOLOGICAL STUDIES AND EVALUATION OF POTENT ENTOMOPATHOGENIC FUNGUS Beauveria bassiana (Bals.)Vuill AGAINST Spodoptera litura (Fab).
 
Creator PATEL, RAHUL S.
 
Contributor PUROHIT, M.S.
 
Subject Unable to Generate Tags patel rahul s.-57914.pdf
 
Description Investigations on “Isolation, identification,
physiological studies and evaluation of potent entomopathogenic
fungus Beauveria bassiana (Bals.)Vuill against Spodoptera litura
(Feb).” were carried out at Bio-control Laboratory, Department
of Entomology, N.M.College of Agriculture, Navsari Agricultural
University, Navsari during 2010-2012.
Microscopic examination and isolation of field
collected larvae of H. armigera yielded whitish fluffy growth of
B. bassiana and produced whitish, single-celled conidia which
was globose to ellipsoid, thin walled and hydrophobic whereas,
mycelium was aseptate and initially transparent with white in
color and later it was converted in to milky white color with
development of dull white colored spore. B. bassiana was found
to be pathogenic to S. litura which exhibited 96.67 per cent mortality on 8th day after treatment.
Sabouraud’s dextrose medium and potato dextrose
medium proved to be superior in the growth and sporulation of B.
bassiana. However, in case of grain media gram, bajara, sorghum
and maiz grain served as a best grain substrate media for growth
and sporulation of B. bassiana.
The best nitrogen sources for growth and sporulation
of B. bassiana were peptone and ammonium phosphate
incorporated in to Sabouraud’s dextrose broth as they produced 66.00 x 108 conidia/ml and 57.00 x 108 conidia per ml,
respectively. Whereas, Sabouraud’s dextrose medium having
glucose and fructose proved to be best carbon sources for growth as well as sporulation (46.33 x 108 and 39 x 108 conidia/ml ) of
B. bassiana. The treatment of temperature 25 ± 2 0C and 30 ± 2 0C
found more congenial for growth and sporulation of B. bassiana as it produced 466.00 X 108 conidia per ml and 392.33 X 108 conidia
per ml, respectively. Whereas, the humidity regimes at 90 ± 5 per cent was most suitable for growth and sporulation (502.00 X 108
conidia/ml) followed by 80 ± 5 per cent RH (450.33 X 108
conidia/ml).
The pH regimes 5.5 and 6.0 proved effective in increasing
growth and sporulation of B. bassiana significantly higher sporulation i.e. 110.67 X 108 conidia per ml and 95.00 X 108 conidia
per ml, respectively.
At 50 minutes of UVexposure treatment, the highest
growth and sporulation was recorded in fungal culture with adjuvant glycerol (32.00 X 108 conidia/ml) and fungus culture with adjuvant tween-80 (25.67 X 108 conidia/ml). The carrier talc found very
effective in increasing the shelf life of B. bassiana even after 6
month.
The inhibition of B. bassiana was cent per cent with
various fungicides viz., mancozeb 75 WP, carbendazim 50 WP,
hexaconazole 5 EC, tridemorph 80 EC, difenconazole 25 EC and
copperoxychloride 50 WP. In case of insecticide it was found that thiamethoxam (803.67 x 106 conidia /ml) was more compatible with B. bassiana which was at par with indoxacarb (758.67 x 106 conidia
/ml) as compared to rest of the insecticide tested.
Symptoms produced by B. bassiana after application on S.
litura noted that the development of small isolated brown to black
spots on host body which cover whole body. These progressed with
death of host and outer proliferation of white mycelium and
ultimately cover the body of host with spore development.
The highest mortality and feeding inhibition of S. litura,
A. janata and E. fraternal was recorded at different doses viz., 1 X 1010, 1X 109 conidia and 1 X 108 conidia per ml. Lethal concentration
of B. bassiana required to kill the 50 per cent larvae of S. litura, A.
janata and E. fraternal were different at each time interval. Fiducial
limit was maximum at 2 day after treatment in case of S. litura, A.
janata and E. fraternal which was decreased as the time laps after
treatment increases.
 
Date 2016-05-10T11:31:27Z
2016-05-10T11:31:27Z
2013-07
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65927
 
Language en
 
Format application/pdf
 
Publisher Navsari Agricultural University