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GENOME WIDE IDENTIFICATION AND CHARACTERIZATION OF HEAT SHOCK FACTOR (Hsf) GENES FROM PIGEON PEA

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Title GENOME WIDE IDENTIFICATION AND CHARACTERIZATION OF HEAT SHOCK FACTOR (Hsf) GENES FROM PIGEON PEA
M.Sc.
 
Creator ALBERT MAIBAM
 
Contributor Kishor Gaikwad
 
Subject Domain, Heat shock response, Phylogenetic, Real Time-PCR.
 
Description T-8801
Pigeonpea [Cajanus cajan (L.) Millspaugh] is one of the important tropical legume
crop providing food and nutritional security to the world population particularly in
southeast Asia. It is a perennial woody shrub, often cross-pollinated crop and
estimated size of its genome packed in 11 chromosomes is 858 Mbp. It belongs to
Fabaceae family. It thrives under annual rainfall less than 650 mm and under moist
soil conditions it can withstand temperatures of (350C) or more. It is a drought
tolerant crop among legumes. Heat shock response is transcriptionally regulated by
conserved heat shock transcription factors (Hsfs). Hsf genes are represented by a large
multigene family in plants. Analysis of Hsf genes were carried out in pigeonpea
genome as no information is available. A total of 23 hsfs genes were predicted by
using FGENESH and labeled as Cchsf. Out of the 23 genes, 14 were selected and
characterized for their presumed structures such as protein domain and motif
organization. The phylogenetic relationships, gene duplication and expression profiling
of Cc Hsf genes were analyzed. Cc Hsf genes were distributed into 8 groups, out of a
total 13 groups. 4 Hsf genes were unrooted from the analysis including Soybean,
Medicago and Arabidopsis. In this study, class A was further subdivided into seven
subclasses according to their bootstrap values and phylogenetic relationship,
designated as A1, A2, A3, A4, A6, A8 and A9. Class B was also subdivided into three
subclasses and designated as B2, B3 and B4. HsfB-3 and Hsf A-6b groups are unique
to this legume. The expression profiling was carried out by semi- quantitative PCR.
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Among the Cc Hsf genes, CcHsfA-1d and CcHsfA-2 were prominently expressed
during the heat stress condition. Relative quantification with Real time PCR shows that
Cc Hsf A-1d is upregulated 2-6 hrs after heat stress but 24 hrs after heat stress it was
down regulated. 42 Hsf gene from soybean were analyzed for the expression in
pigeonpea. Out of 42 Hsf genes, 25 were showing heat induced expression. This
indicates that more hsfs exist in pigeonpea genome and need to be explored once
the complete genome data is available. Overall, result obtained from our study will
contribute to a better understanding of the complexity of the pigeon pea hsf gene family
and provide some clue on the thermotolerance pathway operating in pigeonpea.
 
Date 2016-08-19T09:21:57Z
2016-08-19T09:21:57Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72926
 
Format application/pdf
 
Publisher IARI,NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY