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Isolation, characterization and symbiotic performance of abiotic stress tolerant mungbean (Vigna radiata) rhizobia

KrishiKosh

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Title Isolation, characterization and symbiotic performance of abiotic stress tolerant mungbean (Vigna radiata) rhizobia
 
Creator Mangla
 
Contributor Kukreja, Kamlesh
 
Subject Planting, Root nodulation, Tolerance, Biological development, Application methods, Weather hazards, Rhizobium, Nitrogen, Bacteria, Irrigation
 
Description Abiotic stress, as a natural part of every ecosystem, affects both plants and microorganisms in a variety of ways. Stressed ecosystems are however, the most challenging to realize the benefits from agricultural microorganisms. The present investigation has been undertaken to study the isolation, characterization and symbiotic performance of abiotic stress tolerant mungbean rhizobia. A total of 91 rhizobial isolates were obtained from the healthy nodules of mungbean (Vigna radiata) crop grown in different districts of Haryana (Sirsa, Hisar and Rewari). Four isolates (MS13, MH75, MH77 and MR24) could survive at 1000 mM NaCl concentration. Four rhizobial isolates could grow at 30% PEG concentration (MH75, MH72, MH77 and MR53) and only 2 isolates, MR23 and MS57 could grow at high temperature of 450C. All the rhizobial isolates showed a diminished growth when exposed to the combination of all the three stresses (salinity, drought and high temperature). The EPS production was less under salinity (1000 mM NaCl) and high temperature (450C) stress conditions while under drought stress (30% PEG) conditions the production of EPS was found to be more by the stress tolerant rhizobial isolates than their respective controls. All the different stress tolerant rhizobial isolates were found to accumulate more trehalose when grown under stress conditions than their respective controls. Distinct differences in the whole cell protein pattern were observed between the rhizobial isolates under stress and non stress conditions. New protein bands were observed under the stress conditions than their respective controls. In pot study two rhizobial isolates each from salinity (MH75 and MH77), drought (MH72 and MH75) and high temperature (MR23 and MS57) stress tolerance studies were found to significantly improve all the plant growth parameters of mungbean in comparison to sensitive rhizobial isolate and reference strain even under adverse environmental conditions.
 
Date 2016-02-27T13:14:45Z
2016-02-27T13:14:45Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64465
 
Language en
 
Format application/pdf
 
Publisher CCSHAU