Record Details

Studies on Synergism and Transgenic Resistance against Potato virus Y and Potato virus X

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Studies on Synergism and Transgenic Resistance against Potato virus Y and Potato virus X
Ph.D
 
Creator D.M.J.B. SENANAYAKE
 
Contributor Bikash Mandal
 
Subject fruits, harvesting, mangoes, developmental stages, storage, biological development, acidity, diseases, harvesting date, sugar
 
Description T-8833
Potato virus Y (PVY) of the genus Potyvirus, family Potyviridae and Potato virus X
(PVX), genus Potexvirus, family Alfaflexiviridae are two RNA viruses that together
result in synergistic interaction on mixed infections. PVY and PVX together account for
considerable yield losses in potato. Two well defined strains, PVY-Del66 (N-O strain)
and PVX-ptDel9 that is asymptomatic on potato were used to study their interactions
during mixed infection in Nicotiana benthamiana and Nicotiana tabacum with reference
to symptom expression, level of coat protein (CP) and suppressor genes under high
temperature (26-40°C) and low temperature (5-25°C) conditions. Simultaneous mixed
infection caused severe necrosis and death of plants. Synergistic symptoms in Nicotiana
benthamiana and N. tabacum were more prominent under low temperature conditions
than at high temperature. In low temperature conditions, the coat protein (CP) level of
PVX in N. benthamiana was found to be two-fold higher than the CP of PVY and both
the viruses showed a peak at 28 dpi in single virus infections. However, when PVY and
PVX viruses were inoculated together, the level CP of both the viruses increased and
reached to the peak 7-14 days before (14-21 dpi) than that in single virus inoculations.
The CP level of PVX was higher than PVY in mixed infection in early stages and both
the virus level declined to the similar level at later stage (28 dpi). The levels of
suppressor genes of the same samples revealed that p25 of PVX was higher than HC-Pro
of PVY in single inoculations. Under mixed inoculation of PVY and PVX, expression of
p25 declined to the level of HC-Pro, which co-related to the declining levels of CPs of
PVY and PVX. However, it was found that the expression pattern of CP and suppressor
genes was different in plants when mixed infection was created by inoculation of one
virus followed by the other.
 
Date 2016-06-22T14:22:16Z
2016-06-22T14:22:16Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67724
 
Format application/pdf
 
Publisher IARI,DIVISION OF PLANT PATHOLOGY