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MUTAGENESIS IN OKRA (Abelmoschus esculentus (L.) Moench)

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Title MUTAGENESIS IN OKRA (Abelmoschus esculentus (L.) Moench)
 
Creator Sahu, Gouri Shankar
 
Contributor Ray, D.P.
 
Subject MUTAGENESIS, OKRA
 
Description Seed samples of two morphologically distinct variety of okra (BO-2 and Parbhani Kranti) were treated with gamma rays and ethyl methane sulphonate (EMS). There were seven mutagenic treatments consisting three concentrations of each mutagen and a combination treatment along with one control for each variety. Separate field trials for two varieties were conducted from M1 to M4 generations. Appropriate field plot techniques were followed in each generation and the data collected were subjected to statistical analysis. Selection pressure was applied in M2 and M3 generations and promising micro mutants were selected in each varietals group. The selected micro mutants were evaluated in M4 for yield.
The selected M3 progenies from different mutagenic treatments were grown in M4 generation. Two separate trials were conducted with mutant cultures and parent variety BO-2 and Parbhani Kranti. Each trial consisted of 28 mutant cultures (4 best M3 progenies from each of seven treatments selected on the basis of higher progeny yield in M3 generation) and the parent variety. The trial was conducted in RBD with three replications.
Salient findings
Mutagenic treatments brought about reduction in the mean of M1 characters and the magnitude of reduction was directly related to the dose / concentration of the mutagen. The varieties exhibited differential response to the mutagenic treatments.
The selected mutant cultures were classified into 8 and 5 different groups/clusters in BO-2 and Parbhani Kranti respectively by D2 and canonical analysis in M4 generation which indicated the induction of broad spectrum of micro mutations.
In the present mutation study 13 mutant cultures of BO-2 and ten mutant culture of Parbhani Kranti exhibited significantly higher yield than the parental varieties and recorded less incidence of YVM virus disease. These micro mutants need further evaluation for yield and yield stability.
Conclusion
In the light of the results obtained in the present investigation, it could be concluded that planned mutation breeding may be considered as a useful tool in genetic improvement of okra.
 
Date 2016-12-22T10:33:24Z
2016-12-22T10:33:24Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/92051
 
Language en
 
Relation Th;4100
 
Format application/pdf