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Serdiagnosis and molecular characterization of tomato mosaic virus (TOMV)

KrishiKosh

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Title Serdiagnosis and molecular characterization of tomato mosaic virus (TOMV)
 
Creator Shweta.Ambiger
 
Contributor A.S.Byadagi
 
Subject Plant pathology
 
Description The tomato (Solanum lycopersicum L) is a member of solanaceae family. Among the
viral diseases, tomato leaf curl and tomato mosaic are destructive diseases of tomato in India.
Tomato mosaic disease causes up to 59 percent reduction in the weight of tomato fruit. The
mean incidence of tomato mosaic tobamovirus was 55.98 percent. Till nineteenth century
tomato mosaic disease was considered to be caused by Tobacco Mosaic Virus (TMV). But in
recent years it is called as Tomato Mosaic Virus (ToMV). Hence for confirmation study was
carried out on different aspects viz. Symptomatology, molecular characterization, electron
microscopy, purification of virus, production of antiserum and serodiagnosis. The inoculated
tomato plants developed typical mosaic symptom 10-15 days after inoculation. The electron
microscopic study showed presence of long, rigid rod shape tobamovirus particles. The PCR
successfully amplified the CP-gene from infected tomato leaf samples with specific ToMV
primers. The amplicon of ToMV- CP gene was confirmed by electrophoresis and PCR
product was sent for sequencing. The coat protein had 485 bp and codes for 161 amino acids.
Nucleotide blast results showed that CP gene sequence under study shared highest similarity
with Indian ToMV isolate (97 %) and 95 percent with TMV Indian isolate. With this highest
similarity with TMV isolate, ToMV can be conclude as a strain of TMV, but not a different
virus itself. Polyclonal antiserum against the virus was produced by immunization of rabbit
with partially purified virus preparation. This antiserum had a titer of 1:1024. Double
antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) was standardized by
utilizing g globulins and purified homologous conjugate developed during the study and
was successfully used to detect ToMV in different samples.
 
Date 2016-11-09T17:37:52Z
2016-11-09T17:37:52Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/84744
 
Format application/pdf
 
Publisher UAS, Dharwad