Record Details

Development of plant regeneration protocol(s) from mature and immature embryos in durum and bread wheat

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Development of plant regeneration protocol(s) from mature and immature embryos in durum and bread wheat
 
Creator Bamel, Nisha
 
Contributor Chowdhury, V.K.
 
Subject Plant regeneration, BAP, lBA
 
Description The present study was undertaken to standardize protocol(s) for plant regeneration in selected
bread and durum wheat genotypes. Mature and immature embryos were used as explants. Best medium
found for callus induction from mature and immature embryos of all genotypes was MS basal medium
supplemented with 4 mg/l 2,4-D and 2 mg/l 2,4-D respectively. Bread wheat genotypes were observed to
be more responsive to callus initiation (70-98%) than durum wheat genotypes (50-77%). Frequency of
callus induction was more in calli derived from immature embryos as compared to mature embryos. Shoot
regeneration response was best observed on MS basal medium supplemented with 2 mg/l BAP and 0.1 mg/l
IAA in all durum and bread wheat genotypes. From mature embryos, shoot regeneration response was
maximum in bread wheat genotype WH 1080 (61.2%) and minimum in WH 711 (30.1%) and response was
50-58% in durum wheat genotypes. From immature embryos, shoot regeneration response was 61.4% in
WH 147 and 16.4% in WH 711 of bread wheat genotypes and response was 47-54% in durum wheat
genotypes. Best rooting response was observed on half strength MS basal medium supplemented with 0.5
mg/l IBA. From mature embryos, rooting response observed was 37.7% in PBW 343 and 22.9% in PBW
175 of bread wheat genotypes whereas rooting response was 34-39% in durum wheat genotypes. From
immature embryos, maximum rooting was observed in bread wheat genotype WH 711 (83.3%) and
minimum in PBW 175 (41.8%) whereas in case of durum wheat, rooting response was 87.5% in WH 896
and 55.3% in PDW 291. High plantlet transplanting efficiency was observed in plants regenerated from
mature embryos. Transplantation survival was about 80-90% and 90-95% in all bread wheat genotypes and
durum wheat genotypes respectively. In case of immature embryos, transplantation survival was 70-100%
and 80-90% in bread and durum wheat genotypes respectively.
 
Date 2016-09-10T09:30:59Z
2016-09-10T09:30:59Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76345
 
Language en
 
Format application/pdf
 
Publisher CCSHAU