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MOLECULAR DETECTION AND IDENTIFICATION OF VIRUS CAUSING MOSAIC DISEASE ON COWPEA (Vigna unguiculata L. Walp)

KrishiKosh

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Title MOLECULAR DETECTION AND IDENTIFICATION OF VIRUS CAUSING MOSAIC DISEASE ON COWPEA (Vigna unguiculata L. Walp)
 
Creator PAVITHRA, B S
 
Contributor Prameela, H A
 
Subject diseases, fungi, chickpeas, biological development, planting, biological phenomena, genotypes, extraction, irrigation, pathogens
 
Description Survey conducted during 2012-13 in eight districts of Southern Karnataka to
assess the incidence of mosaic disease of cowpea revealed the occurrence of disease in
the range of 11.66 to 36.66 per cent. Highest disease incidence was recorded in
Bengaluru urban (36.66%) and least disease incidence in Ramanagar district (11.66%).
The virus was readily transmitted through sap and also by aphid Aphis craccivora. The
transmission varied from 88.00 to 96.00 per cent. The transmission of the virus through
aphids varied between 44.00 to 64.00 per cent. The per cent seed transmission of the
virus varied from 33.33 to 47.07. The virus transmitted to Green gram and Groundnut.
The purified preparation of the virus under electron microscopic revealed the presence of
long flexuous filamentous particles approximately measuring 952nm in size. DACELISA
of symptomatic samples from field showed positive reaction to Potyvirus group
antisera. RT-PCR technique for detection of bean common mosaic virus isolates was
standardized. PCR product of approximately 750bp corresponding to BCMV CP was
amplified using coat protein gene specific primers. The virus isolates (BCMV GKVK 1
and GKVK 2) 91-95.6 per cent homology with already known bean common mosaic
virus isolates which includes BICMV strains also. The two isolates share 91-95 per cent
nucleotide and 92-98 per cent amino acid sequence homology with other BCMV strains
from different geographical locations. Among 21 genotypes screened under field
condition, MFC-09-19 showed immune, 14 showed resistance reaction and five showed
moderately resistance reaction. Out of 60 cowpea genotypes screened under green house
condition by sap inoculation nine showed immune, 12 were resistant, 19 showed
moderately resistance reaction.
 
Date 2016-11-14T13:05:52Z
2016-11-14T13:05:52Z
2013-07-24
 
Type Thesis
 
Identifier TH-10540
http://krishikosh.egranth.ac.in/handle/1/85397
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore