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STUDIES ON MOLECULAR CHARACTERIZATION OF SOME Prunus ROOTSTOCKS

KrishiKosh

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Title STUDIES ON MOLECULAR CHARACTERIZATION OF SOME Prunus ROOTSTOCKS
 
Creator VERMA, ASHISH
 
Contributor KAUR, RAJINDER
 
Subject toxicity, proteins, acidity, biological phenomena, diseases, aromatic compounds, enzymes, organic acids, carbohydrates, papayas
Prunus rootstocks, molecular characterization ,fingerprinting ,CTAB method
 
Description ABSTRACT
In the present studies on molecular characterization and fingerprinting of some Prunus
rootstocks, DNA from fresh, young and healthy leaves of 18 Prunus rootstocks was isolated following
CTAB method (Doyle and Doyle, 1987) with some modifications. Out of a total of 20 SSR primers used,
only 16 were able to amplify the genomic DNA .Eight microsatellite primers were found to be
monomorphic and eight were found to be polymorphic. The eight polymorphic primers gave a total of 13
polymorphic bands out of which only five were found to be unique for rootstocks ‘Cadaman’, ‘GF 677’,
‘Wild apricot’, ‘Manicot’ and ‘Gisela 5’. Similarity matrix using NTSYSpc ver. 2.02h and dissimilarity
matrix using DARwin5 ver. 5.0.155 CIRAD was constructed and it ranged from 0.533 to 1.00 and 0.00 to
0.34, respectively. The rootstocks ‘Wild peach’ and ‘Paja’ and ‘GF 31’ and ‘Julior’ were found to be
identical. Dendrogram based on UPGMA method divided the rootstocks into two broad clusters ‘A’ and
‘D’ and the rooted tree based on Neighbour-Joining method of DARwin5 ver. 5.0.155 CIRAD also
divided the rootstocks as per the results of the dendrogram. The PIC value for all the eight polymorphic
primers was calculated and the mean PIC value was found to be 0.38. The primer IISRS-8 gave highest
PIC value (0.67) and the lowest PIC value (0.09) was obtained with primer IISRS-9. Polymorphism was
found to be 43.33%. From all the data obtained, it can be concluded that more number of informative
primers would be incorporated in the study to molecular characterize and fingerprint these rootstocks.
 
Date 2017-01-03T12:26:14Z
2017-01-03T12:26:14Z
2010
 
Type Thesis
 
Identifier 47716
http://krishikosh.egranth.ac.in/handle/1/94127
 
Language en
 
Format application/pdf