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Nutritional Histopathology of Aflatoxicosis and its Possible Effects on Immunosupression in Labeo Rohita

KrishiKosh

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Title Nutritional Histopathology of Aflatoxicosis and its Possible Effects on Immunosupression in Labeo Rohita
 
Creator Varuna Baglodi
 
Contributor Jayaraj, E.G.
Pai, R.
Benakappa, S.
Gangadhar Gowda
Rajesh, K.M.
 
Subject mycotoxins, biological phenomena, livestock, acidity, research methods, bacteria, organic acids, biological development, fungi, toxicity
 
Description Ph.D. thesis
The study on nutritional histopathology of aflatoxicosis and its possible effects on immunosupression in Labeo rohita were investigated in 3 phases. In the first phase of aflatoxicosis induction, dietary inclusion of aflatoxin (AFB1) on the survival and growth performance and gut histology of Labeo rohita were investigated for 130 days in outdoor cement tanks. In the first phase the basal diet in all the trials contained around 30% protein with different levels of AFB1 at 50 ppb (F1), 100 ppb (F2), 150 ppb (F3) and 0 ppb AFB1 (F0) respectively in triplicates. The mean survival was found to be more than 90% in all the treatments and control group without significant difference in the survival rate of fish. The routine observations indicated no visible/ external changes or unusual behaviour in any of the fish fed with AFB1 supplemented diets. No significant difference (P>0.05) was evident in both mean weight gain and mean length of fish between different treatment groups and control. The mean weight gain of fishes in 150 ppb treated AFB1 showed a fall in growth at 130 days of feeding indicating the initiation of aflatoxicosis and the resultant reduction in the growth. Similarly, no significant difference was evident in specific growth rate (SGR) (P>0.05) and feed conversion ratio (FCR) (P>0.05) between the treatments. Loss of normal cellular architecture of villi was observed in different AFB1 fed groups compared to control diet. Infiltration of RBC and thinning of musculosa increased with increase in the concentration of AFB1. Initiation of transformation of goblet cells to macrophages was observed in 150 ppb fed groups. In the second phase of amelioration of aflatoxicosis, ameliorating effect of different toxin binders like MOS and lactic acid incorporated feed over control feed was investigated for 30 days on immunological parameter like neutophile count, gut histology was investigated. In the second phase of amelioration of aflatoxicosis, the basal diet in all the trials contained around 30% protein with different levels of toxin binders like 0.15% MOS (T1), 3% lactic acid (T2) and contol diet without MOS/lactic acid (T0). The respiratory burst activity i.e. super oxide anion production (NBT assay) of fish varied significantly (P
 
Date 2016-07-04T10:09:32Z
2016-07-04T10:09:32Z
2015-05-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/68385
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar