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STUDIES ON CALLUS INDUCTION AND TRANSFORMATION PROTOCOL IN SELECTED RICE (Oryza sativa L.) GENOTYPES USING PARTICLE INFLOW APPARATUS

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Title STUDIES ON CALLUS INDUCTION AND TRANSFORMATION PROTOCOL IN SELECTED RICE (Oryza sativa L.) GENOTYPES USING PARTICLE INFLOW APPARATUS
 
Creator REVATHI, P
 
Contributor NAGESHWAR RAO, T
 
Subject STUDIES, CALLUS, INDUCTION, TRANSFORMATION, PROTOCOL, SELECTED, RICE , GENOTYPES, USING, PARTICLE , INFLOW, APPARATUS
 
Description of different hormonal concentrations and combinations for callus induction and
growth and to determine the best source of explant [leaf pieces, root segments and mature
seeds (dehusked)] for callus induction and growth in four rice genotypes, viz., BPT-5204,
Swarna, Surekha and Tellahamsa. Studies were also conducted to determine the
transformation frequency of direct explants and calli derived from mature seeds of the
four rice genotypes using particle inflow apparatus.
Callus initiation was observed in all the explants of the four rice genotypes with all
the treatments MS + 2,4-D 2 mg/l + kinetin/BA 0.5 mg/l was found to be better for callus
induction and regeneration capacity was also higher. Among the different explants,
mature seed (dehusked) was found to be more responsive for callus induction and growth.
Tellahamsa was found to be superior followed by Swarna for various in vitro parameters.
The differences observed in callus induction among different explants and between the
four rice genotypes may be attributed to the differences in endogenous hormonal
concentrations among different explants and differences in genetic constitution of
genotypes, respectively.
Transformation of direct seedling explants and calli derived from mature seeds of the four genotypes was attempted using reporter gene (gus) by particle bombardment. gus
expression was observed in all the explants of four genotypes but, with variable levels.
The success in plant transformation using this device is dependent on several factors
including (i) pressure of helium gas, (ii) distance between the micro carrier and macro
carrier and the target, (iii) genotype of the target species and (iv) arrangement of target
tissues. So, various parameters need to be optimized for successful transformation. The
present study thus indicate that genetic transformation in rice can be successfully
achieved by optimizing various parameters for particle bombardment but further work
needs to be done on stable integration of the introduced gene and regeneration of plantlets
from transformed cells or tissues.
 
Date 2016-08-22T10:23:02Z
2016-08-22T10:23:02Z
2003
 
Type Thesis
 
Identifier ACHARYA N.G. AGRICULTURAL UNIVERSITY RAJENDRA NAGAR, HYDERABAD - 500 03 0
http://krishikosh.egranth.ac.in/handle/1/73238
 
Language en
 
Format application/pdf