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Effect of Bioagents and Their Metabolites on Sclerotium rolfsii Sacc. Of Groundnut

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Title Effect of Bioagents and Their Metabolites on Sclerotium rolfsii Sacc. Of Groundnut
 
Creator Siddanagouda R.Radder
 
Contributor S.S.Adiver
 
Subject Plant Pathology
 
Description Groundnut (Arachis hypogaea L.) is an annual legume and an important oil seed crop
of India. Stem rot caused by Sclerotium rolfsii is an economically important disease on
groundnut. The fungus was isolated from infested plants showing typical stem rot symptoms.
Bioagents like Trichoderma spp. (T. harzianum), T. viride, T. virens and T. koningii) were
isolated from soil by serial dilution technique. This techniques was also used to isolate from
talc formulated products of University of Agricultural Sciences, Dharwad and Project
Directorate of Biological Control. Among the six media tested potato dextrose agar supported
maximum growth of the Trichoderma spp. and produced maximum quantity of metabolites
and among the Trichoderma spp. grown T. harzianum (Dwd) isolate recorded highest
biomass. Sabourd’s agar medium was least suited to the growth of the Trichoderma spp.
T. harzianum (Dwd) inhibited the maximum mycelial growth in dual plate technique.
Least inhibition of mycelial growth of S. rolfsii was recorded by T. koningii. Culture filtrates
of Trichoderma spp were found effective when they are fractionated at high speed
centrifugation than at low speed. Both top and bottom fractions of culture filtrates of
bioagents at high speed centrifugation were on par with each other. The inhibitory
activity of the culture filtrates remained unaffected even after sterilization. The S. rolfsii
inhibitory substances produced by the Trichoderma spp in culture filtrates were found to be
thermo stable.
Among the formulations tested the talc formulated culture filtrate was effective than
lignite and wheat flour as a seed dresser. In soil applicant formulations, culture filtrate
formulated with Farm Yard Manure was found effective to S. rolfsii with enhanced seed
germination.
Higher concentrations of the formulation were found to be useful in promotion of
seed germination by way of suppressing the activity of S. rolfsii.
 
Date 2016-10-24T18:40:40Z
2016-10-24T18:40:40Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81645
 
Format application/pdf
 
Publisher UAS, Dharwad