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STANDARDIZATION OF SEED TESTING PROCEDURES IN SEVEN MEDICINAL PLANTS

KrishiKosh

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Title STANDARDIZATION OF SEED TESTING PROCEDURES IN SEVEN MEDICINAL PLANTS
 
Creator SIVA JYOTHI, T
 
Contributor MEENA KUMARI, K.V.S
 
Subject STANDARDIZATION, SEED, TESTING, PROCEDURES, SEVEN, MEDICINAL, PLANTS
 
Description In medicinal plants, the productivity and expansion for large scale production is
restricted due to non-availability of quality seeds. A good quality seed is a pre-requisite
to obtain healthy plant. Hence, standardized seed testing procedures are essential for
obtaining accurate and reproducible seed testing results and to know the planting value of
seed lots offered for sale. It is now recognized that greater precision is required for
standard germination testing. This recognition has led to improved standardization and
uniformity in comparative tests.
The procedures for testing the seeds of medicinal plants viz., Ashwagandha
(Withania somnifera), Dulagandi (Mucuna pruriens), Isabgol, (Plantago ovata), Kalmegh
(Andrographis paniculata), Podapatri (Gymnema sylvestre), Senna (Cassia angustifolia)
and Tulasi (Ocimum sanctum), are not available in the international rules for seed testing
(ISTA, 2006) and National rules provided in the seed testing manual. It revealed that the
standardized procedures for physical purity and germination in medicinal plants have not
been provided in the rules for seed testing. Hence, this study was undertaken to
standardize the procedures for physical purity, germination and seed vigour tests for the
seven medicinal plants.
The germination percentage was varied with testing medias and genotypes. Top of
paper method was very effective in recording higher germination for Isabgol(87.9%),
Kalmegh(88.2%), Senna(94.4%) and Ashwagandha(74.7%). While, Podapatri gave
maximum germination(53.7%) on between paper method. Sand(86.0%) and bp(85.0%)
methods for Dulagandi and sand method(81.7%) for Tulasi were found very effective to
obtain higher germination.
Seedling vigour index on length basis was differed with testing medias and genotypes.
Top of paper method for Ashwagandha and Kalmegh, sand for Dulagandi, Podapatri and
Tulasi, between paper for Isabgol and Senna were effective to obtain higher seedling
vigour index on length basis. Soil was found very effective for obtaining higher seedling
vigour index II for Ashwagandha, Dulagandi, Isabgol and Kalmegh, between paper for
Podapatri and Senna. Sand for Tulasi gave maximum seedling vigour index on dry
weight basis.
Vigour tests of first count and final count for Dulagandi and Kalmegh; brick gravel
for Dulagandi and Senna; cool germination test for Isabgol and Dulagandi; paper
exhaustion test for Dulagandi and Isabgol; speed of germination for Senna and Isabgol;
mean germination time for Senna, Isabgol and Dulagandi and field emergence for
Dulagandi and Kalmegh were very effective for obtaining many number of vigorous
seedlings which were superior over methods.
The germination percentage gradually decreased with increase in period of
accelerated ageing. Such decline was marginal in Dulagandi and Kalmegh which shows
resistant to seed deterioration by accelerated ageing and considered as a good storers.
Rapid deterioration by accelerated ageing was observed in Podapatri, Tulasi and Isabgol
which were considered as poor storers. Moderate decline in germination and vigour in
Ashwagandha and Senna which were considered as moderate storers.
The Submitted and working sample sizes were determined based on number of
seeds and seed weight, where the germination, physical purity, seedling length, seedling
dry weight and seedling vigour index were recorded optimum. 15,000 seed weight (30g)
for Ashwagandha, Isabgol (22g), Dulagandi (37kg) Podapatri (140g), Kalmegh (18 g),
Senna (35g ) and Tulasi (5g) considered as submitted sample and its one tenth is as
working sample, which were recorded optimum values in seed quality parameters. Oven
dry method was effective for estimating the moisture content of seed in all medicinal crop
seeds as against silica gel desiccator.
It was concluded that top of paper method for Ashwagandha, Kalmegh, Isabgol
and Senna; between paper and sand media for medium sized seeds (Podapatri); between
paper and sand methods were effective for large seeds(Dulagandi) for recording higher
germination. Sand method in Tulasi were found effective in detemining the germination
percentage.
 
Date 2016-08-05T14:13:49Z
2016-08-05T14:13:49Z
2008
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71061
 
Language en
 
Relation D8298;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD