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STUDIES ON YELLOW MOSAIC VIRUS RESISTANCE IN GREENGRAM USING MORPHOLOGICAL AND MOLECULAR MARKERS

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Title STUDIES ON YELLOW MOSAIC VIRUS RESISTANCE IN GREENGRAM USING MORPHOLOGICAL AND MOLECULAR MARKERS
 
Creator JYOTHSNA NAND, M
 
Contributor ANURADHA, CH
 
Subject developmental stages, genotypes, sowing, planting, genetics, integrated pest management, diseases, yields, biological phenomena, polymorphism
 
Description Mungbean, (Vigna radiata (L.) Wilczek) is an excellent source of easily digestible
proteins with low flatulence which complements the staple rice diet in Asia. The protein is
comparatively rich in lysine, an amino acid that is deficient in cereal grains but, cereals are
rich in methionine, cystine and cystein, the sulphur bearing amino acids. The average yield
of mungbean is very low, not only in India, but in entire tropical and subtropical Asia. Plant
viruses are responsible for a significant proportion of crop diseases that result in serious
crop losses. Among the various viral diseases, Yellow Mosaic Disease (YMD) is reported
to be the most destructive viral disease caused by Yellow mosaic virus (YMV) and it leads
to severe yield reduction. The advancements in the field of biotechnology and molecular
biology such as marker assisted selection and genetic transformation can be utilized in
developing Mungbean yellow mosaic virus (MYMV) resistant mungbean.
The present investigation is on screening of mungbean genotypes against Mungbean
yellow mosaic virus (MYMV) and identification of sources of resistance and to study the
genetic diversity based on morphological characters and molecular markers. The genetic
diversity studies were carried out during rabi 2012-2013 at experimental field of college
farm in Acharya N.G. Ranga Agricultural University (ANGRAU), with 50 genotypes to
elicit the information on nature and extent of the genetic variability, heritability, genetic
advance, trait correlation, path analysis and genetic divergence. For molecular diversity
studies 54 genotypes employing SSR primers are carried out. Observations were recorded
on 11 characters viz., days to initial flowering, days to 50% flowering, number of branches
per plant, number of pods per plant, number of seeds per pod, days to full maturity, 100-
seed weight, seed yield per plant, pod length, 50% podding and percent of disease
incidence.
The analysis of variance revealed significant differences among the genotypes for
all the characters studied indicating that the genotypes represented wide variability. The
genotypic coefficients of variation for all the characters studied were lesser than the
phenotypic coefficients of variation indicating the modifying effects of the environment in
association with the characters at genotypic level. High PCV and GCV estimates were
observed for number of pods per plant, seed yield per plant. High heritability along with
high genetic advance as percent of mean was observed for number of pods per plant,
number of seeds per pod, seed yield per plant indicating the role of additive genes in
governing the inheritance of these traits and could be improved through selection.
The results of multivariate analysis of morphological data revealed the presence of
considerable genetic diversity among the 50 genotypes studied which grouped into four
clusters as per D2 analysis. Out of 11 characters studied, percent disease incidence
(32.65%) contributed maximum towards divergence followed by number pods per plant
(16.49%), number of seeds per pod (11.43%), seed yield per plant (6.86%), 100 seed
weight(6.37%) and days to full maturity (6.12 %), days to initial flowering (5.80%),
number of branches per plant (5.47%), days to 50% flowering (4.90%), 50% podding
(2.94%) and pod length (0.98%).
Crosses between genotypes LGG-542, PM-112, LGG-581 (II), MH-521, IPM/02-
03, MGG-350, IPM/02-19 (III) and LGG-537 (IV) are expected to exhibit high heterosis
and might result in high yielding segregants with desired traits. Cluster IV showed the
highest mean value for seed yield per plant. Cluster II recorded highest mean value for
number of pods per plant. Cluster IV showed maximum mean value for 100 seed weight.
Cluster IV recorded the highest mean value for number of seeds per pod. Cluster III
recorded highest mean value for number of branches per plant, Cluster II recorded highest
mean value in pod length and 50% podding.
The correlation studies indicated that days to initial flowering, days to 50%
flowering, no. of branches per plant, no. of pods per plant, no. of seeds per plant, days to
full maturity, 100 seed weight (g), pod length (cm) and 50% podding are positively and non
significantly correlated with seed yield and simultaneous improvement of these characters
along with seed yield is possible. The path analysis for seed yield revealed that number of
seeds per pod, pod length, 50% podding and 100-seed weight has high positive direct
effects and they would directly increase seed yield.
In molecular diversity studies of 54 SSR primers employed only 14 primers detected
polymorphism while remaining primers were monomorphic. The neighbour-joining tree
developed based on weighted average for dissimilarity matrix using DARWIN, grouped the
54 genotypes into three clusters. Cluster-I contains 25 genotypes and the similarity
coefficients ranged from 20-50% from similarity matrix, Cluster-II contains 15 genotypes
and the similarity coefficients ranged from 22-62%, Cluster-III contains 14 genotypes and
the similarity coefficients ranged from 16-45%.
Both morphological traits and SSR markers were highly effective in estimating
genetic diversity and distinguishing genotypes in mungbean. Direct selection for yield
improvement through traits such as number of seeds per pod, pod length, 50% podding and
100 seed weight would be rewarding. Lowest diseased genotypes PM-113, LGG-545,
LGG-564, IPM/02-10, LGG-548, LGG-496, LGG-538, LGG-580, LGG-544, LGG-551,
LGG-537, LGG-577, MH/3-18, LGG-522, LGG-491, LGG-542, PM-115, LGG-558, LGG-
582, PUSA-06-072, LGG-572, LGG-460, LGG-578, LGG-575, LGG-540, LGG-581,
LGG-568, LGG-543, LGG-569 and LGG-479 can be used to breed for resistance to
MYMV.Sufficient variability exists in the mungbean genotypes which are potential to be
used in selection of suitable parents for breeding, genetic mapping studies and QTL
analysis.
 
Date 2016-06-13T12:13:41Z
2016-06-13T12:13:41Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67261
 
Language en
 
Relation D9312;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY