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BIOCHEMICAL CHANGES IN MICROPROPAGATED BANANA (Musa sp.) cv. DWARF CAVENDISH (AAA) DUE TO ARBUSCULAR-MYCORRHIZAL FUNGI (AMF) INOCULATION DURING EX VITRO ACCLIMATIZATION

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Title BIOCHEMICAL CHANGES IN MICROPROPAGATED BANANA (Musa sp.) cv. DWARF CAVENDISH (AAA) DUE TO ARBUSCULAR-MYCORRHIZAL FUNGI (AMF) INOCULATION DURING EX VITRO ACCLIMATIZATION
 
Creator Vartika Srivastava
 
Contributor Prof. Anand Kumar Singh
 
Subject Micropropagated Banana, Biochemical changes, Arbuscular-mycorrhizal fungi (AMF), ex vitro acclimatization
 
Description The present investigation entitled “Biochemical changes in micropropagated banana (Musa sp.) cv. Dwarf Cavendish (AAA) due to arbuscular-mycorrhizal fungi (AMF) inoculation during ex vitro acclimatization” was carried out during 2008-2010 in Department of Horticulture, Institute of Agricultural Sciences, BHU, Varanasi. On the basis of the findings of present investigation, it is concluded that for surface sterilization of shoot-tips double sterilization with mercuric chloride (0.1%) for 5 minutes followed by quick dip in ethanol showed best results for minimum contamination and maximum survival of explants. Further, for culture establishment and multiplication 6 mgL-1 BAP + 1 mgL-1 IAA was found to be the best combination showing maximum shoot induction as well as multiplication. For rooting, half strength MS media + 1 mgL-1 IAA was found to be effective for maximum rooting, reduction in days to root induction and number of roots per micro-shoot.
The results with root colonization of micropropagated banana plantlets inoculated with two individual and mixed arbuscular-mycorrhizal strains reveal that AMF are potential inoculants for averting transplant shock experienced by plantlets during acclimatization under net-house conditions. In nutshell, AMF colonization occurred rapidly in about 20 days of inoculation which had a positive impact on photosynthetic rate, stomatal conductance and RWC when plantlets were experiencing desiccation during acclimatization. During nutritional stress, AMF plantlets maintained higher N, P and K levels. This indirectly led to higher chlorophyll levels, accelerated photosynthetic rate and stomatal conductance at the termination of the experiment. Due to these changes mycorrhized plantlets showed better growth responses as compared to control under moisture stress. This study suggests that changes in levels of total chlorophyll, total soluble sugar, starch, proline, phenol and enzymes effectively overcame transplanting shock along with moisture stress and enhanced ex vitro survival under low input conditions. Physiological and biochemical changes brought about by AMF association rendered the plantlets to be more resistant against microbial infection and water stress conditions. Thus, major shortcomings of tissue cultured plants viz., poor absorption and transportation and sensitivity to microbial infection could be ameliorated with desirable traits of AMF association. Thus, these microorganisms could be used in future alternative biotechnologies for banana production systems. On the basis of present investigation, it may be concluded that hardening period of banana plantlets could be reduced and made more economic with the use of mycorrhizal fungi during planting in ex vitro conditions. Biopriming with AMFs not only minimizes extra input in terms of water, fertilizer, pesticides and sophisticated structures like mist chambers but also improves resistance of mycorrhized tissue cultured plants to water and nutritional stress which are generally faced by such plants during transport and during growth periods.
 
Date 2016-09-08T21:26:30Z
2016-09-08T21:26:30Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76179
 
Language en
 
Relation P-0002;
 
Format application/pdf
 
Publisher Institute of Agricultural Sciences, Banaras Hindu University