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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF SELECTED COLEUS SPECIES AND MUTANTS OF Coleus forskohlii Briq.

KrishiKosh

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Title MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF SELECTED COLEUS SPECIES AND MUTANTS OF Coleus forskohlii Briq.
 
Creator PRASANNA, HEGDE
 
Contributor VASUNDHARA, M
 
Subject wines, sugar, alcohols, acidity, fruits, fermentation, storage, organic acids, phenols, productivity
 
Description Coleus is an important medicinal plant containing a diterpinoid
called forskolin in its tuberous roots. In the present study 3 Coleus
species, 11 mutants and a variety of C. forskohlii were characterized
through morphological and molecular markers.
In morphological study entries differed significantly for different
morphological and yield characters. MV5 was superior with respect to
plant height (60 and 120 DAP), number of branches (at 180 DAP), fresh
and dry root weight, number of roots, root length, total biomass and
harvest index. Aisiri (MV7) recorded maximum number of branches (60
days and 120 DAP) and a forskolin content of 0.74%. SV2 recorded
highest forskolin content of 0.76%. Among the various species under the
study Coleus vettiveroides differed from others for both morphological
and yield characters.
In the molecular diversity study 20 RAPD primers generated 293
bands, of which 13 were polymorphic. Different Coleus species and
mutants under study differed from Coleus vettiveroides in dendrogram,
generated by using banding pattern of decamers. Principle component
analysis with 2 dimensional and 3 dimensional figures also showed the
same clustering as of the dendrogram. In the distance matrix higher
genetic distant of 50% was observed in between SV1 and Coleus
aromatics var. variegated and least distance of 7% in MV9 and K-8.
SCAR was developed from the primer RAPD-4. Thus, it can be concluded
that although the mutants were morphologically alike, a narrow genetic
difference was existed between them.
 
Date 2016-12-15T12:56:47Z
2016-12-15T12:56:47Z
2011-08-24
 
Type Thesis
 
Identifier Th-10084
http://krishikosh.egranth.ac.in/handle/1/90299
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore