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Effect of sulphur supply on sulphur and nitrogen assimilating enzymes and protein quality in developing soybean [Glycine max (L.) Merrill.] seeds

KrishiKosh

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Title Effect of sulphur supply on sulphur and nitrogen assimilating enzymes and protein quality in developing soybean [Glycine max (L.) Merrill.] seeds
Effect of sulphur supply on sulphur and nitrogen assimilating enzymes and protein quality in developing soybean [Glycine max (L.) Merrill.] seeds
 
Creator Kaur, Gurpreet
 
Contributor Sharma, Sucheta
 
Subject sulphur, proteins, sowing, amino acids, soybeans, enzymes, inorganic acid salts, nitrogen, planting, acidity
 
Description The present study was conducted to evaluate the effect of two sulphur sources viz. gypsum and single super phosphate (SSP) under field conditions at three levels 10, 20 and 30 kg S ha-1 on sulphur and nitrogen assimilating enzymes and protein quality in developing soybean [Glycine max (L.) Merrill] seeds. The activities of nitrogen assimilating enzymes viz. nitrate reductase, glutamate synthase, glutamine synthetase activities were maximum in developing seeds at 45 days after podding (DAP) in all the treatments. Glutamate
dehydrogenase activity decreased whereas activities of sulphur metabolizing enzymes i.e. serine acetyl transferase and o-acetyl serine thiol lyase increased from 30 to 60 DAP in developing seeds in control and different treatments of sulphur. Gypsum/SSP applied at different dose rates significantly increased activities of nitrogen and sulphur metabolizing
enzymes as compared to control. Total soluble proteins, free amino acids, methionine,
cysteine and glutathione contents increased from 30 to 60 DAP in developing seeds and
maximum increase was observed with higher doses of gypsum/SSP. In mature seeds, protein
quality parameters i.e. total soluble proteins, free amino acids, methionine content and seed
storage protein fractions increased with both sulphur sources whereas trypsin inhibitor
activity showed the reverse trend. Maximum increase in total soluble proteins, free amino
acids and methionine content was observed in treatment with SSP @ 30 kg S ha-1. Among the
various treatments, highest sulphur accumulation and the maximum decrease in N:S ratio was
observed in treatment with gypsum @ 20 kg S ha-1. The albumin and globulin content in
developing seeds increased with various treatments of gypsum/SSP. Among the glycinin and
-conglycinin, glycinin showed higher response to different sulphur treatments resulting in
increased 11S/7S ratio, which is desirable as glycinins are rich in sulphur containing amino
acids. The average secondary structure of proteins did not change during seed-filling stage as
revealed by Fourier transform infrared spectroscopy.
 
Date 2016-04-08T14:42:08Z
2016-04-08T14:42:08Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65410
 
Language en
 
Format application/pdf
 
Publisher PAU