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Molecular characterization and development of mapping populations for construction of genetic map in dolichos bean (Lablab purpureus L. (Sweet))

KrishiKosh

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Title Molecular characterization and development of mapping populations for construction of genetic map in dolichos bean (Lablab purpureus L. (Sweet))
 
Creator S. C, Venkatesha
 
Contributor P. H, Ramanjini, Gowda
 
Subject sowing, genetics, planting, yields, developmental stages, genotypes, biological phenomena, selection, crossing over, polymorphism
 
Description Fieldbean {Lablab purpureus L. (Sweet)} is an important pulse crop in India. Though
it is being one of the ancient crops with rich genetic diversity and adaptability to varied
edaphic factors, it has not attained the level of agricultural significance as expected. To
facilitate the breeding activities the present study was conducted in relation to marker and
population development with the aim of providing tools for the study of Indian fieldbean.
The morphological characterization, development of F2 mapping populations, Molecular
diversity of selected fieldbean using COS legume markers and molecular characterization of
224 germplasm accessions were carried out at GKVK, UAS, Bengaluru. The screening of
selected fieldbean accessions to identify polymorphic markers and their validation in F2
mapping populations were carried out in John Innes Centre, Norwich, UK. The diversity
analysis showed that Fieldbean genotypes of Indian origin grouped into one single cluster
while genotypes from African origin grouped into second cluster. Principal component
analysis for 71 traits in 224 germplasms revealed 81% of variation in the population by first
four principal components.
High rate of amplification of cowpea genic SSR primers in fieldbean showed high
marker transferability between these two legumes. Four polymorphic markers were identified
in parental genotypes when separated on 5% horizontal polyacrylamide gel while these were
monomorphic on 2% agarose gel. The sequencing of 18 genic fragments among nine parental
genotypes, revealed 60 SNPs and 16 InDels. Using SNPs and InDels information of genic
fragments allele specific markers were developed. Four molecular marker systems viz.,
sequencing of genic fragments, SNPs, allele specific PCR markers and InDel markers were
used successfully to genotype the F2 populations of HA4×CPI31113 and HA4×CPI60216
crosses and they exhibited 1:2:1 ratio. F2 populations of HA4×CPI31113 and HA4×CPI60216
crosses exhibited 50% and 60% transgressive segregants for total seed yield per plant,
respectively.
 
Date 2016-05-19T09:11:42Z
2016-05-19T09:11:42Z
2012-10-22
 
Type Thesis
 
Identifier Th-10406
http://krishikosh.egranth.ac.in/handle/1/66090
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru