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Expression of White Spot Syndrome Viral Coat Proteins and Study of their Immunogenic Potential

KrishiKosh

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Title Expression of White Spot Syndrome Viral Coat Proteins and Study of their Immunogenic Potential
 
Creator Girisha, S.K
 
Contributor Indrani Karunasagar
Karunasagar, I.
Venugopal, M.N.
Shamsunder, B. A.
Maragal, M.M.
Krishna Bhatt, C.H.
 
Subject biological phenomena, proteins, diseases, recombination, genes, fruits, amino acids, cloning, application methods, electrophoresis
 
Description Ph.D. Thesis
White spot syndrome virus following its first appearance in Taiwan in the 1990s has
spread to all the shrimp farming countries including India. Due to its ability to spread
quickly and cause 100% mortality in 3-10 days causing large scale economic losses, this
virus has threatened the very sustainability of shrimp aquaculture. Therefore, this virus has
attracted vigorous research interest in the last one decade. Despite the application of
numerous strategies, the problem of WSSV infection still remains unabated. Unlike in
higher animals, where many microbial diseases have been controlled by vaccination, till
recently vaccination has not been a well accepted strategy in invertebrates owing to the
assumed lack of adaptive immune system. However, the recent discovery of “quasi immune
response” in invertebrates including shrimps has opened up new frontiers towards
development of vaccine-based protection strategies against WSSV infection in shrimps.
Though the whole and inactivated viral particles are known to enhance disease resistance,
the practical approach has been to identify viral components interacting with host immune
components and stimulate adaptive immune response. Envelope proteins of virus are thus
considered potential vaccine candidates, since these first come in contact with the host
immune system. The present study was initiated with the aim of identifying useful vaccine
candidate proteins of WSSV that would serve to protect P. monodon against infection by
this virus. To achieve this, the genes coding for 4 envelope proteins were cloned and
expressed in E. coli. The efficacy of these recombinant proteins as vaccines to protect P.
monodon against WSSV infection was investigated.
 
Date 2016-07-05T09:40:40Z
2016-07-05T09:40:40Z
2007-06-14
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/68445
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar