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Chemical and biological characterization of hepatoprotective efficacy of leaf extracts isolated from Murraya koenigii and Phyllanthus niruri using in vivo and in vitro model systems
KrishiKosh
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| Title |
Chemical and biological characterization of hepatoprotective efficacy of leaf extracts isolated from Murraya koenigii and Phyllanthus niruri using in vivo and in vitro model systems
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| Creator |
Shah, Pallavi
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| Contributor |
Singh, S.P.
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| Subject |
biochemistry, characterization, leaves, plant extracts, isolation, Murraya koenigii, Phyllanthus niruri, in vitro, in vivo, medicinal plants
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| Description |
Thesis-PhD
The present study was undertaken to determine the hepatoprotective efficacy of Murraya koenigii and Phyllanthus niruri hydroethanolic leaf extracts against paracetamol and alcohol induced damage in both in vivo rat and in vitro HepG2 cell line models. Different leaf extracts were prepared and their phytochemical analysis was carried out to determine the active principles present in them. The AWE (Alcohol Water Extract), WE (Water Extract) and AE (Alcoholic Extract) of P. niruri possessed higher content of total phenolics in comparison to that of M. koenigii with AWEs possessing highest content of phenolics for both the plants. The AWEs had the highest total flavonoid content as well i.e. 61.8 and 65 mgCatechin equivalents/g of leaf extract for M. koenigii and P. niruri, respectively. Examination of antioxidant activity by DPPH free radical scavenging assay showed P. niruri extracts had higher antioxidant potential than M. koenigii extracts with AWEs for both the plants to possess the highest scavenging potential. Tannic acid (TA), a known antioxidant was quantified using HPLC in M. koenigii extracts with AE showing the highest content of TA (288ppm). HPLC quantification of phyllanthin, a lignan known for its hepatoprotective potential was done for all P. niruri extracts with WE showing the highest content (223ppm). The GC MS analysis of plant extracts further detected the presence 40 compounds in AWE of M. koenigii and 41 compounds in AWE of P. nruri. Oral administration of alcohol (5mL/kg) for one week followed by paracetamol (1g/kg) for three days produced liver damage in rats as manifested by the significant (P |
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| Date |
2016-10-26T11:57:02Z
2016-10-26T11:57:02Z 2014-01 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/82092
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| Language |
en
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| Format |
application/pdf
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| Publisher |
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
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