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ASSESSMENT OF GENETIC DIVERSITY IN RICE (Oryza sativa L.) GERMPLASM EMPLOYING YIELD RELATED TRAITS AND MOLECULAR MARKERS

KrishiKosh

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Title ASSESSMENT OF GENETIC DIVERSITY IN RICE (Oryza sativa L.) GERMPLASM EMPLOYING YIELD RELATED TRAITS AND MOLECULAR MARKERS
 
Creator LALITH KIRAN, BH
 
Contributor LAKSHMI NARAYANA REDDY, V
 
Subject genetics, rice, genotypes, yields, planting, genetic structures, biological phenomena, dna, polymorphism, heritability
 
Description Rice (Oryza sativa L.) is the main staple food crop of Asia and is being consumed by more than half of the population of the developing countries in the world. Genetic diversity is pre-requisite for any crop improvement programmes as it helps in the development of superior recombinants and selection of parents having wider variability for different characters. Therefore, there is a need to diversify the genetic base of improved rice cultivars. Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Sixty rice genotypes were used to study genetic diversity based on characters (8) SSR markers (20) morphological and molecular levels. The morphological data was recorded for 8 characters viz., plant height, number of panicles, number of chaffy grains per panicle, number of filled grains per panicle, hundred seed weight (g), panicle length (cm), total yield per plant (g) and spikelet fertility (%). The PCV and GCV were high for number of panicles, number of filled grains per panicle, plant height and total yield. Heritability estimates
were high for plant height, number of panicles and total yield traits under study. Genetic advance as percent of mean was high for the traits viz., plant height, number of panicles, total yield and spikelet fertility. The studies on genetic divergence through Tochers method indicated the presence of considerable diversity by forming four clusters with a wide range of intra-cluster distance. The diversity was more for the clusters IV and II indicating their importance in contribution to the genetic diversity. The cluster IV recorded higher mean value for plant height, number of filled grains per panicles and total grain yield/plant and lower mean were recorded for number of chaffy grains per panicle. Correlation analysis intrepetated that plant height, number of spikelets per panicle and hundred seed weight recorded a positive and significant association with total yield, spikelet fertility and panicle length respectively whereas number of chaffy spikelets per panicle recorded a negative and significant association with spikelet fertility. Microsatellites which showed a wide variation with respect to PIC value ranged 0.69 (RM22250) to 0.86 (RM562) with a range of 0.78 and Nei's genetic diversity index (He) values varied considerably among the loci from 0.71 at RM22250 to 0.89 at RM562 with an average of 0.59. Molecular data was analysed using DARwin software indicated that genotypes NLR33359 and NLR0106 cluster IV showed higher variability both phenotypically and genotypically which can be used as parental lines for further breeding programme.
 
Date 2016-06-13T12:15:16Z
2016-06-13T12:15:16Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67263
 
Language en
 
Relation D9313;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY